LOCUS       sequence-62548-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Shigetomi E, Bushong EA, Haustein MD, Tong X, Jackson-Weaver O, 
            Kracun S, Xu J, Sofroniew MV, Ellisman MH, Khakh BS
  TITLE     Imaging calcium microdomains within entire astrocyte territories and
            endfeet with GCaMPs expressed using adeno-associated viruses.
  JOURNAL   J Gen Physiol. 2013 Apr 15.
  PUBMED    23589582
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gen 
            Physiol. 2013 Apr 15."
COMMENT     Sequence Label: pZac2.1 gfaABC1D-tdTomato
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(112..135)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(310..329)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     579..597
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 cggccgcccg ggtcgactct agaatctact tgtacagctc gtccatgccg tacaggaaca
       61 ggtggtggcg gccctcggag cgctcgtact gttccacgat ggtgtagtcc tcgttgtggg
      121 aggtgatgtc cagcttggtg tccacgtagt agtagccggg cagttgcacg ggcttcttgg
      181 ccatgtagat ggtcttgaac tccaccaggt agtggccgcc gtccttcagc ttcagggcct
      241 ggtggatctc gcccttcagc acgccgtcgc gggggtacag gcgctcggtg gaggcctccc
      301 agcccatggt cttcttctgc attacggggc cgtcgggggg gaagttggtg ccgcgcatct
      361 tcaccttgta gatcagcgtg ccgtcctgca gggaggagtc ctgggtcacg gtcaccagac
      421 cgccgtcctc gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttct
      481 tgtaatcggg gatgtcggcg gggtgcttca cgtacgcctt ggagccgtac atgaactggg
      541 gggacaggat gtcccaggcg aagggcaggg ggccgccctt ggtcaccttc agcttggcgg
      601 tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt
      661 tcatggagcc ctccatgcgc accttgaagc gcatgaactc tttgatgacg gccatgttgt
      721 tgtcctcgga ggaggcggtg ccggagctgc cgctgccggt gctgccggtg ccatgcc
//
