LOCUS       sequence-62552-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral shRNA vector (pGIPz based) for inducible knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, 
            Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi 
            RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, 
            Elledge SJ
  TITLE     The pINDUCER lentiviral toolkit for inducible RNA interference in 
            vitro and in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 
            10.1073/pnas.1019736108. Epub 2011 Feb 9.
  PUBMED    21307310
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019736108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-03-1- 1"; volume: "108"; issue: "9"; pages: "3665-70"
COMMENT     Sequence Label: pInducer10-mir-RUP-PheS
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    130..163
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gtgttttgtg aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat
       61 tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta
      121 gacggatcca taacttcgta taatgtatgc tatacgaagt tatgccacca tgtctagact
      181 ggacaagagc aaagtcataa acggagctct ggaattactc aatggtgtcg gtatcgaagg
      241 cctgacgaca aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca
      301 cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc gagatgctgg acaggcatca
      361 tacccacttc tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa
      421 gtcataccgc tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg
      481 cccaacagag aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg
      541 cttctccctg gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg
      601 cgtattggag gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga
      661 ttctatgccc ccacttctga gacaagcaat tgagctgttc gaccggcagg gagccgaacc
      721 tgccttcctt ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga
      781 aagcggcggg ccgaccgacg cccttgacga ttttgactta gacatgctcc cagccgatgc
      841 c
//
