LOCUS       sequence-62555-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral shRNA vector (pGIPz based) for inducible knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, 
            Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi 
            RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, 
            Elledge SJ
  TITLE     The pINDUCER lentiviral toolkit for inducible RNA interference in 
            vitro and in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 
            10.1073/pnas.1019736108. Epub 2011 Feb 9.
  PUBMED    21307310
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019736108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-03-1- 1"; volume: "108"; issue: "9"; pages: "3665-70"
COMMENT     Sequence Label: pInducer10-mir-RUP-PheS
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..752
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     ncRNA           803..896
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     896..912
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     897..913
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gctgttttga cctccataga agacaccggg accgatccag cctaccggtc gccaccatga
       61 gcgagctgat caaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc
      121 accacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga
      181 agatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct
      241 tcatgtacgg cagcaaagcc ttcatcaacc acacccaggg catccccgac ttctttaagc
      301 agtccttccc tgagggcttc acatgggaga gaatcaccac atacgaagac gggggcgtgc
      361 tgaccgctac ccaggacacc agcttccaga acggctgcat catctacaac gtcaagatca
      421 acggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacacgc ggctgggagg
      481 ccaacaccga gatgctgtac cccgctgacg gcggcctgag aggccacagc cagatggccc
      541 tgaagctcgt gggcgggggc tacctgcact gctccttcaa gaccacatac agatccaaga
      601 aacccgctaa gaacctcaag atgcccggct tccacttcgt ggaccacaga ctggaaagaa
      661 tcaaggaggc cgacaaagag acctacgtcg agcagcacga gatggctgtg gccaagtact
      721 gcgacctccc tagcaaactg gggcacagat gatgcggccg caagccttgt taagtgctcg
      781 cttcggcagc acatatacta tgttgaatga ggcttcagta ctttacagaa tcgttgcctg
      841 cacatcttgg aaacacttgc tgggattact tcttcaggtt aacccaacag aaggctcgag
      901 gtcgacggta tcgataagct tg
//
