LOCUS       sequence-62557-e         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Stegmeier F, Rape M, Draviam VM, Nalepa G, Sowa ME, Ang XL, McDonald
            ER 3rd, Li MZ, Hannon GJ, Sorger PK, Kirschner MW, Harper JW, 
            Elledge SJ
  TITLE     Anaphase initiation is regulated by antagonistic ubiquitination and 
            deubiquitination activities.
  JOURNAL   Nature. 2007 Apr 19;446(7138):876-81.
  PUBMED    17443180
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2007-04-19"; volume: "446(7138)"; pages: "876-81"
COMMENT     Sequence Label: pCS2-USP44-HIS
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(178..198)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(250..270)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(280..297)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(313..330)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(377..395)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(378..395)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 atgctcttca atatatcttc cacaggcaac atgggagcag ctaaggcaag cccaaatgga
       61 ctcggtcgtg ttgcagtcca cacagtgcca tttctgaggg ttgaggctgg aatggtcttg
      121 agcaagctgc agctgcccaa catgtttgca cgtatccatt gctagcatgg gccggccgcc
      181 ctggaagtac aggttctcgc tgctgccggg ccggggcttg ccggcatcgg cgccggcatc
      241 cacggtgccg ctttgaaaat ataaattttc ccggcctgca tggtggtgat gatggtgctg
      301 agaacctcta gcatggtgat ggtgatggtg tgatcctcga gccatggatc ctgcaaaaag
      361 aacaagtagc ttgtattcta tagtgtcacc taaatcaagc ttgctccgac gtccccaggc
      421 agaatggcgg ttccctaaac gagcattgct tatatagacc tcccattagg cacgcctacc
      481 gcccatttac gtcaatggaa cgcccatttg cgtcattgcc cctccccatt gacgtcaatg
      541 gggatgtact tggcagccat cgcgggccat ttaccgccat tgacgtcaat gggagtactg
      601 ccaatgtacc ctggcgtact tccaatagta atgtacttgc caagttacta ttaatagata
      661 ttgatgtact gccaagtggg ccatttaccg tcattgacgt caataggggg cgtgagaacg
      721 gatatgaatg ggcaatgagc catcccattg acgtcaatgg tgggtggtcc tattgacgtc
      781 aatgggcatt gagccaggcg ggccatttac cgtaattgac gtcaatgggg ggaggcgcca
      841 tatacgtcaa taggaccgcc catatgacgt caataggaaa gaccatatat agagaccatt
      901 gacgtcaatg ggggagtggc tatgggcggt attaggaagc cccatatatg gtatatggga
      961 ccgcccattg ggaaggggct atctacgtca atagga
//
