LOCUS       sequence-62561-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Stegmeier F, Rape M, Draviam VM, Nalepa G, Sowa ME, Ang XL, McDonald
            ER 3rd, Li MZ, Hannon GJ, Sorger PK, Kirschner MW, Harper JW, 
            Elledge SJ
  TITLE     Anaphase initiation is regulated by antagonistic ubiquitination and 
            deubiquitination activities.
  JOURNAL   Nature. 2007 Apr 19;446(7138):876-81.
  PUBMED    17443180
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2007-04-19"; volume: "446(7138)"; pages: "876-81"
COMMENT     Sequence Label: pCS2-USP44
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(204..222)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(205..222)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 cttcaatata tcttccacag gcaacatggg agcagctaag gcaagcccaa atggactcgg
       61 tcgtgttgca gtccacacag tgccatttct gagggttgag gctggaatgg tcttgagcaa
      121 gctgcagctg cccaacatgt ttgcacgtat ccattgctag catgggccgg cctgatcctg
      181 caaaaagaac aagtagcttg tattctatag tgtcacctaa atcaagcttg ctccgacgtc
      241 cccaggcaga atggcggttc cctaaacgag cattgcttat atagacctcc cattaggcac
      301 gcctaccgcc catttacgtc aatggaacgc ccatttgcgt cattgcccct ccccattgac
      361 gtcaatgggg atgtacttgg cagccatcgc gggccattta ccgccattga cgtcaatggg
      421 agtactgcca atgtaccctg gcgtacttcc aatagtaatg tacttgccaa gttactatta
      481 atagatattg atgtactgcc aagtgggcca tttaccgtca ttgacgtcaa tagggggcgt
      541 gagaacggat atgaatgggc aatgagccat cccattgacg tcaatggtgg gtggtcctat
      601 tgacgtcaat gggcattgag ccaggcgggc catttaccgt aattgacgtc aatgggggag
      661 gcgccatata cgtcaatagg accgcccata tgacgtcaat aggaaagacc atatatagag
      721 accattgacg tcaatggggg agtggctatg ggcggtatta ggaagcccca tatatggtat
      781 atgggaccgc ccattgggag gggctatcta cgtcaatagg aaaacccata tatggaatac
      841 tatatggcat agggccaata catagtattt gaacctggcc aatagccata ttggcatagg
      901 gccatattg
//
