LOCUS       sequence-62571-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Nevozhay D, Zal T, Balazsi G
  TITLE     Transferring a synthetic gene circuit from yeast to mammalian cells.
  JOURNAL   Nat Commun. 2013 Feb 5;4:1451. doi: 10.1038/ncomms2471.
  PUBMED    23385595
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013-02-5"; volume: "4:1451. doi"; pages: " 
            10.1038/ncomms2471"
COMMENT     Sequence Label: pDN-D2irC6kwh
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        96..143
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             162..560
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    718..839
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(755..774)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     809..828
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(888..904)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(888..904)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
       61 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt
      121 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag cctttgtctc
      181 aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg
      241 aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca
      301 atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg
      361 ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct
      421 tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca
      481 tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg
      541 gttatgtgtg ggagggctaa gcacttcgtg gccgaggagc aggactgaca cgtgctacga
      601 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
      661 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
      721 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
      781 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
      841 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
      901 cctgtgtga
//
