LOCUS       sequence-62572-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Nevozhay D, Adams RM, Murphy KF, Josic K, Balazsi G
  TITLE     Negative autoregulation linearizes the dose-response and suppresses 
            the heterogeneity of gene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 
            10.1073/pnas.0809901106. Epub 2009 Mar 11.
  PUBMED    19279212
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0809901106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-03-31- 31"; volume: "106"; issue: "13"; pages: "5123-8"
COMMENT     Sequence Label: pDN-G1Gh
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..732
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 ccggatccta ttaaaatgtc taaaggtgaa gaattattca ctggtgttgt cccaattttg
       61 gttgaattag atggtgatgt taatggtcac aaattttctg tctccggtga aggtgaaggt
      121 gatgctactt acggtaaatt gaccttaaaa tttatttgta ctactggtaa attgccagtt
      181 ccatggccaa ccttagtcac tactttcggt tatggtgttc aatgttttgc gagataccca
      241 gatcatatga aacaacatga ctttttcaag tctgccatgc cagaaggtta tgttcaagaa
      301 agaactattt ttttcaaaga tgacggtaac tacaagacca gagctgaagt caagtttgaa
      361 ggtgatacct tagttaatag aatcgaatta aaaggtattg attttaaaga agatggtaac
      421 attttaggtc acaaattgga atacaactat aactctcaca atgtttacat catggctgac
      481 aaacaaaaga atggtatcaa agttaacttc aaaattagac acaacattga agatggttct
      541 gttcaattag ctgaccatta tcaacaaaat actccaattg gtgatggtcc agtcttgtta
      601 ccagacaacc attacttatc cactcaatct gccttatcca aagatccaaa cgaaaagaga
      661 gaccacatgg tcttgttaga atttgttact gctgctggta ttacccatgg tatggatgaa
      721 ttgtacaaat aactcgagta agcttggtac cgcggctagc taagatccgc tctaaccgaa
      781 aaggaaggag ttagacaacc tgaagtctag gtccctattt atttttttat agttatgtta
      841 gtattaagaa cgttatttat atttcaaatt tttcttttt
//
