LOCUS       sequence-62573-e         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Nevozhay D, Adams RM, Van Itallie E, Bennett MR, Balazsi G
  TITLE     Mapping the environmental fitness landscape of a synthetic gene 
            circuit.
  JOURNAL   PLoS Comput Biol. 2012;8(4):e1002480. doi: 
            10.1371/journal.pcbi.1002480. Epub 2012 Apr 12.
  PUBMED    22511863
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Comput
            Biol."; date: "2012"; volume: "8(4):e1002480. doi"; pages: " 
            10.1371/journal.pcbi.1002480. Epub 2012 Apr 12"
COMMENT     Sequence Label: pDN-T2dMFot
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      216..405
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(477..494)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggggtcca
       61 gctgacgcct tggatgactt tgatttggat atgttgcctg cagatgctct tgacgatttc
      121 gacctagaca tgctacccgc cgacgcatta gatgactttg atttagatat gttgccaggt
      181 taactcgagt aagcttggta ccgcggctag ctaagatccg ctctaaccga aaaggaagga
      241 gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga
      301 acgttattta tatttcaaat ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac
      361 attatactga aaaccttgct tgagaaggtt ttgggacgct cgaagatcca gctgcattaa
      421 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
      481 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
      541 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
      601 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      661 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      721 g
//
