LOCUS       sequence-62588-e        1212 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Sehnke PC, Laughner B, Cardasis H, Powell D, Ferl RJ
  TITLE     Exposed loop domains of complexed 14-3-3 proteins contribute to 
            structural diversity and functional specificity.
  JOURNAL   Plant Physiol. 2006 Feb;140(2):647-60. Epub 2006 Jan 11.
  PUBMED    16407442
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2006-02"; volume: "140(2)"; pages: "647-60. Epub 
            2006 Jan 11"
COMMENT     Sequence Label: pET15b-At 14-3-3 phi
FEATURES             Location/Qualifiers
     source          1..1212
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atggcggcac caccagcatc atcctcggcg agggaagagt tcgtgtacct cgcaaagctc
      121 gcagagcaag cggaacgtta cgaagaaatg gttgaattca tggaaaaagt cgctgaagcc
      181 gttgacaaag acgaactcac cgtcgaagaa cgtaatctcc tctccgtcgc ttacaaaaac
      241 gtaatcggcg ctcgtcgtgc ttcctggaga atcatctctt ccattgaaca aaaagaagag
      301 agtcgtggta acgatgacca tgtgaccacg atccgtgatt acagaagcaa gatcgaatct
      361 gagttatcga aaatctgtga cggtattctt aagcttcttg atactagact tgttcctgct
      421 tctgctaatg gagattctaa ggttttttac cttaagatga agggagatta tcataggtat
      481 ttggctgagt ttaagactgg tcaagagagg aaagatgctg ctgaacatac tctcaccgct
      541 tacaaagctg ctcaggatat tgctaatgct gaattggctc caacgcatcc gattcgtctt
      601 ggtttggctt tgaatttctc tgtgttttac tatgagattc ttaattctcc agatcgtgct
      661 tgtaatctcg ctaagcaggc gtttgatgaa gccattgctg agttagatac tcttggagag
      721 gagtcataca aggatagtac cttgatcatg cagcttcttc gtgacaatct tactctctgg
      781 acttctgaca tgcaggacga aagtccggag gagattaaag aagcagcagc accaaagcct
      841 gctgaagaac agaaggagat ctaagtttgt ggtttctttt ttcagtattc ttaagttatg
      901 caattataat tatgtttctg cttggtgttt attttctaat ctttcctttg ctatccccga
      961 agcttctaag gccgtctaat tcttgtcctt tgtttgcttt tctcaatcgt ggatgctact
     1021 ggatttctgt tttgttttac ttcttctctt gattgattgc atttcccggc ttatgccttg
     1081 tacttgaaac tcagttttct ctcttattaa tgatttgcca ttcaccaaaa aaaaaccccg
     1141 aattccagct gagcgccggt cgctaccggt ccggctgcta acaaagcccg aaaggaagcg
     1201 atgtgctgcg gg
//
