LOCUS       sequence-62589-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Sehnke PC, Laughner B, Cardasis H, Powell D, Ferl RJ
  TITLE     Exposed loop domains of complexed 14-3-3 proteins contribute to 
            structural diversity and functional specificity.
  JOURNAL   Plant Physiol. 2006 Feb;140(2):647-60. Epub 2006 Jan 11.
  PUBMED    16407442
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2006-02"; volume: "140(2)"; pages: "647-60. Epub 
            2006 Jan 11"
COMMENT     Sequence Label: pET15b-At 14-3-3 nu
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atgtcgtctt ctcgggaaga gaatgtgtac ttagccaagt tagctgagca agctgaacgt
      121 tatgaggaaa tggttgagtt catggagaaa gttgcaaaga ctgttgacac cgatgagctt
      181 actgtcgaag agagaaacct cttgtctgtt gcttacaaga acgtcattgg tgctaggaga
      241 gcttcctgga ggatcatatc ttccattgaa cagaaggaag aaagcagagg aaacgatgat
      301 catgtttcca ttatcaagga ctacagagga aagatcgaaa ctgaactcag caaaatctgt
      361 gatggaatac tcaatcttct ggattctcac cttgttccca ctgcatcttt ggccgagtcc
      421 aaagtctttt acctcaaaat gaaaggagat taccacaggt accttgctga gtttaagact
      481 ggagctgaga ggaaagaagc tgctgagagc actctggttg cttacaagtc agctcaggat
      541 attgcacttg ctgatttagc tcctactcat ccgattagac tgggacttgc tcttaacttc
      601 tctgtcttct actacgagat tctcaactca cctgatcgtg cctgcagtct cgcaaaacag
      661 gcttttgatg aggccatttc tgagctggat acattaggag aagaatcata caaagacagt
      721 acgttgataa tgcaacttct ccgtgacaat ctgacccttt ggaactctga catcaatgat
      781 gaggcgggcg gtgatgagat caaggaggcg tcaaaacatg agccggaaga ggggaaacca
      841 gctgagacag ggcagtgacc aggatcccgg ctgctaacaa agcccgaaag gaagctgagt
      901 tggctgctgc caccccctga gatatctatc gcatgaaaga
//
