LOCUS       sequence-62590-e        1058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Sehnke PC, Laughner B, Cardasis H, Powell D, Ferl RJ
  TITLE     Exposed loop domains of complexed 14-3-3 proteins contribute to 
            structural diversity and functional specificity.
  JOURNAL   Plant Physiol. 2006 Feb;140(2):647-60. Epub 2006 Jan 11.
  PUBMED    16407442
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2006-02"; volume: "140(2)"; pages: "647-60. Epub 
            2006 Jan 11"
COMMENT     Sequence Label: pET15b-At 14-3-3 epsilon
FEATURES             Location/Qualifiers
     source          1..1058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atggagaatg agagggaaaa gcaggtttac ttggctaagc tctccgagca aaccgaaaga
      121 tacgatgaaa tggtggaggc gatgaagaaa gttgctcagc ttgatgtgga gctaactgtg
      181 gaagagagga atcttgtatc tgtagggtac aagaatgtga ttggtgcaag gagagcatca
      241 tggagaatac tatcttccat tgagcagaag gaagagtcca agggaaatga tgaaaatgtc
      301 aagaggctta agaattatcg taagagagtt gaagatgagc ttgctaaagt ttgtaatgac
      361 atcttgtctg tcattgataa gcatctcatt ccatcgtcta acgctgtgga gtcaactgtc
      421 tttttctaca aaatgaaagg agattactat cgctatcttg cggagttcag ttctggtgct
      481 gaacgcaagg aagctgcaga tcagtctctt gaagcatata aggctgctgt tgctgctgca
      541 gagaatggtt tggcacccac acatccagtt agacttggct tggcgttgaa cttttcagtt
      601 ttctactatg agatcttgaa ctctcccgaa agcgcatgcc aattggctaa gcaagcattc
      661 gatgatgcaa ttgctgaact tgacagcctc aacgaggaat catacaaaga cagcactctt
      721 attatgcagc tacttagaga caatctcacc ttgtggactt cagaccttaa tgaggaagga
      781 gatgagagaa ccaaaggtgc tgatgagcct caagatgaga actaaatcct ctgtgagaag
      841 agaaacgact cttgctgcat cctgaatctt gaagtgaaga cagtaagtgt cgttgtttgt
      901 tactcgaatg tgtattttta tctatgtctt ctgatggtgt ttttccagat tcttgaactt
      961 ttcacacaca acactggcgt tgcgtatctg gatcggctgc taacaaagcc ggaaagggaa
     1021 gctgagttgg ctgcttgcca cccgcctacn nantacat
//
