LOCUS       sequence-62597-e       15665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15665)
  AUTHORS   Steiner FA, Talbert PB, Kasinathan S, Deal RB, Henikoff S
  TITLE     Cell-type-specific nuclei purification from whole animals for 
            genome-wide expression and chromatin profiling.
  JOURNAL   Genome Res. 2012 Apr;22(4):766-77. doi: 10.1101/gr.131748.111. Epub 
            2012 Jan 4.
  PUBMED    22219512
REFERENCE   2  (bases 1 to 15665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.131748.111"; journalName: "Genome Res"; date: "2012-04";
            volume: "22"; issue: "4"; pages: "766-77"
COMMENT     Sequence Label: pFS16 Pmyo-3 NPP-9 mChBioFLAG
FEATURES             Location/Qualifiers
     source          1..15665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(2632..2643)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             12213..12920
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(12358..12376)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     12626..12645
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             12999..13064
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(13477..13497)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(13477..13495)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(13516..13532)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13516..13532)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13529..13551)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13540..13556
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13564..13570,13571..13588,13589..13594))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13609..13630
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13747..13764)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13918..14506)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13998..14017)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14677..15468,15469..15537))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15300..15319
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15538..15642)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgcaaag cttcagtaaa agaagtagaa ttttatagtt ttttttctgt
      721 ttgaaaaatt ctccccatca atgttctttc aaataaatac atcactaatg caaagtattc
      781 tataacctca tatctaaatt cttcaaaatc ttaacatatc ttatcattgc tttaagtcaa
      841 acgtaacatt aaaaaaaatg ttttggaaaa tgtgtcaagt ctctcaaaat tcagtttttt
      901 aaaccactcc tatagtccta tagtcctata gttacccatg aaatccttat atattactgt
      961 aaaatgtttc aaaaaccatt ggcaaattgc cagaactgaa aatttccggc aaattgggga
     1021 accggcaaat tgccaatttg ctgaatttgc cggaaaaacg gtaattgccg aaagtttttg
     1081 acacgaaaat ggcaaattgt ggttttaaaa tttttttttt tggaaatttc agaatttcaa
     1141 ttttaatcgg caaaactgta ggcatcctaa gaatgttcct acatctattt tgaaaagtaa
     1201 gcgaattcta tgaaaatgtc taaagaaaat ggggaaacaa tttcaaaaag gcacagtttc
     1261 aagtgtttcc gaattatact aaatccctct aaaaacttcc ggcaaattga tatccgtaaa
     1321 agagcaaatc cgcatttttg ccgaaaatta aaatttccga caaatcggca aaccggcaat
     1381 ttggcgaaat ttgccggaag gcaattgccg cccacccctg ttccagaggt tcaaactggt
     1441 agcaaagctc aaaatttctc aaattctcca attttttttt gaattttggc agtgtaccaa
     1501 aatgacattc agtcatattg gtttattata gatttattta gataaaatcc taaatgattc
     1561 tacctttaaa gatgcccact ttaaaagtaa tgactcaaac ttcaaattgc tctaagattc
     1621 tattgaatta ccatcttttc ctctcatttt ctctcactgt ctatttcatc acaaattcat
     1681 ccctctctcc ctctctttct ctctccctct ctctctttct ctttgctcat catctgtcat
     1741 tttgtccgtt cctctctctg cgccctcagc gttccccaca ctctctcgct tctcttttcc
     1801 tagacgtctt cttttttcat cttcttcagc cctttttcgc cattttccat ctctgtcaat
     1861 cattacggac gacccccatt aatttttgga tgtgctctgc gagcaagagc acacggaact
     1921 catttatttt ttgttggaat ttctttttca ttttagttag ttaaaaggtt attcagtgca
     1981 aaaattgatt ttttgctctt ttggtacatt agaggggttt gaaagttttc cataaaaagg
     2041 gtttccggag taaaaaatct aaaaattttt acaaacattt ccaaaaattt tgggaacata
     2101 tcttttcaaa aactcaaaaa aaaacgcaaa catcataaat ttaatcaatt aattggatgc
     2161 gttcaaatat tttaaaaata tttttgaatt gcatatttct ttactcataa aaaaattatt
     2221 aatgtttaag taaaaaaaat atttcaggat tttaaacaaa aactgtaaat cccataaaac
     2281 agtgttattt tttagttatt tttagtaatt atttgtgaat taaaaacaca ttttttttca
     2341 aaatattcct tgaataaata aaattgaaag tgaattcaaa atatttcacc aattttacaa
     2401 aatttgaaaa tttgaaaaaa tattttctac caaattgtgc tcaaaaaata ttattaagtt
     2461 cccaggaaat ttatgatgaa aacttggaat atttttggta aaattttctc aaaaaattga
     2521 aagttgtcaa aaacacacta acactatacc tcgcggcata gaaaaaactg gtggccgaat
     2581 tttttaaacc aatttaaatt aaatatattt ttgcactttg tacaaaaaaa tttgaagttc
     2641 ccaggaaatt catacggaaa tattttccag aacttttata atttttattt taattctcaa
     2701 aatttacaat aaaaacttgg aaacctgaaa tttgcttaaa gaataaccct atcccccacg
     2761 gcctagaaaa tactggtggc cgaatttttt ccgcagccac accacctcta atctccagag
     2821 cactctccaa aattcccata atccccaaaa ttttcagcta caacagcata tatgaaggca
     2881 gagcaacaac aacaatcgat cgcacccggc tcggcaacct tcccgtctca ggtgaggaag
     2941 acgcagagaa atgagagaga gagcgtgttt atgcacgcag tatactaact cctgatatcc
     3001 aatttcatat atcgatatgc ttgctttctt ttcttctacg ttgaaattgt ttgaactttt
     3061 ctcaaaaaac gcggatttta gagagactta gagttgctcc aaataaattt aatttgcaat
     3121 tttgcactta ctattagaat ctacttttat agcgctaaaa tgaccgaata acacagtaga
     3181 ttttaaaaga aaattttaaa aattattttt ttgtgcagtc aaatttcgct agtttttgcc
     3241 actcattttt gaacatttgt atatgtgcct ttcggtggtt caatcagggg tggcacgtgg
     3301 tgtcagagtg ttccatttcg gtttgatcta cgttgatcta caaaaaatgc gggagaagag
     3361 acgcagagtt ctcaactgat ttcacatggt tatgaaccag gggtgttcgg caaatttgcc
     3421 gtttgccgag ctgggcaaat tttgagattt gccgcacacc ccttttatga acgtgctgac
     3481 gtcacatctt tttggacaaa gaattcccgc attttttgta tatcaaaccg tgatgggaca
     3541 gcctggcacc acgtggggtt ggcggcaaat gattttttcc gacaaatcgg caaattggca
     3601 aattgccgga atttaaagtt tctggcaaat tgttgatttg ccgaaaaaaa atttcaaaaa
     3661 ggcacagttt taagtgtttc cgtcttataa aatatttctc taaaaatttc cggaaaacgg
     3721 aaaattgccg gaaatacaaa gtttcgtcaa atcggcaaac ctgcaatttg ccgaacggca
     3781 actgccgcca acacctggtt cccaaaaaag cgtgcactgt ttgaatatta aaaaaaaaat
     3841 tcacacaaaa ataataaatt gaaataggct aaattcacat ctatgtacaa aaattgcatg
     3901 ccagcaccgg tcaattttct caattacttt ttgtctattt ttgccgaaca taatttgctg
     3961 tttccttggc agaatacaga aaactctttt ttttttcaga tgccccgacc cccaccggta
     4021 accgaacagg ctataaccac cgaggcggag cttctcgcga aaaatcaaat tacaccaaat
     4081 gatgtgctag ctcttccagg aatcactcaa ggtaagtgaa tatttgctct attgataaac
     4141 gcgccgtgta ctccacgtgg acaaacatat caaattttca ggattcctat gctccccatc
     4201 ggcaaacgtc tataacattg agttcaccaa attccaaatc cgtgacctcg acacggaaca
     4261 cgtgctcttc gaaattgcaa aaccggaaaa tgagacggaa gagaatctgc aggcgcaagc
     4321 cgaatcggca agatatgtcc gatatcgatt tgcgccgaat tttctgaaat taaagacggt
     4381 cggcgcgacg gtcgagttca aggtcggcga tgtgccgatc acacattttc gaatgattga
     4441 acggcacttt ttcaaggatc gtttattgaa atgctttgat tttgaatttg gattctgtat
     4501 gccaaattcg agaaacaact gtgaacatat ctatgaattt ccacaacttt cacaacaact
     4561 cagtgagttt gaagcgggaa aaattatttg agctcatttt gtcaatagag cgaattttct
     4621 aggtttttaa aaataaaata aatattgaca aaaagctgcc ctacagtagt tcaaaatgtg
     4681 aaaacttgga ggaaattcga gttttctctg agaagttttt ggcgggtaat tcaaattttc
     4741 tgagaaaaaa attagccgcg agaattcaaa ttttctgaga aaattatttg acgggaaatt
     4801 caaaatttct ggaaatgtat tgaacatttg agaaattttg ttaaaaacta cattaaaccg
     4861 ataaaaaagt tcgcaaattt tttttaagtg ctcatggtgc atcgaagaga agaattcttg
     4921 ccaaaactag cccatgaaat taaaaattat cgcggaaaaa aactgactaa ttggaaagta
     4981 aaatgaaatt tgcaaaaaaa aaatatgttt ttttgttgta aaattaaaaa aaaaactgta
     5041 tattttcagt ggacgacatg atcaataatc caaacgagac ccgctccgat agcttctatt
     5101 ttgtggagaa taagctcgta atgcacaata aggccgacta ctcgtatgat gcataatttc
     5161 ccgccgatgg aataaaacta ataatttttc tcgcaattta tagaaatcat gtgattttcc
     5221 taccgctaaa tttaaaattt cctaattttt cactatattt ctcacaaaat gcattccgaa
     5281 aactctcatt tttcagcttt tcaatacaaa tcccaataaa tgatttttca aatataatgt
     5341 tctcaattga tatgttttat ttgaatgttg gaaaaaacca taaaattaaa aaaaacttga
     5401 attattcaaa aataattcgg cccttttaca aaaaattatc ataaaaatta aatttggaaa
     5461 atctgtgatt tgtcgctttg ccggaagttt ccaattccgg caactaaccg atttgccgat
     5521 tagctagaaa tttcaattcc gacaatgtgc cgctttgccg atttgccgga aatttccaat
     5581 tccggcaatt aacggacttg ccgattagct agaaatttaa ctttagataa aacttctcaa
     5641 caaaatcaaa ttggaaagtc agcgatttgc cgctttgccg gaagttttca gttccggcat
     5701 ctaaccgatt tgccgatttg ccaaaaatgt caattccgaa aaactgccga tttgccgctt
     5761 tgtcggaaat ttcaattccg acaaattgcc gatttgccga tttgccgaac attccaattc
     5821 cgaccatttg tcgatttgcc ggaagttacc aattccggca actaacggat ttgccgattt
     5881 gccggaaaaa aattgtttgt cgccaaccct gatttaaatg actaatcatg ttgcaaaaga
     5941 taactgcttg catcaaaaat ccgatcattg gcattcccaa catcttcttc atccaaaaca
     6001 ccaccctttt cttccactaa tcatgaattc ccgaccaatt gttatcagta ggagaagcct
     6061 ccttttatct cattatcagc aattgccttc ttcttcttct tctttgaata ccatttgaat
     6121 ggagatatca tttacataaa tctgccgccg agattgtgaa gcaggaggaa aagctgaaga
     6181 tactgatatt atatgtatat ttcattattc tttttccaca aaattttccg acatttaatt
     6241 ccacttcact ttcatcgtct ctttttcccc cattgtcgcc cccactcatt tgtcttcttc
     6301 ttcaaatacg ggtcaatcac tttccgtttt ttattcattt tttgttatgt tcggcttaac
     6361 aaatttgaag tattgttaaa attctctagt tgcggccgct ctagaactag tacctactgg
     6421 cgcgccgtga ttatagtctc tgttttcgtt aattttgaat tttgcttgat aaggctgcaa
     6481 caaagatcag gttgacatat attttcagta atttatttta acctgtactc tatcactgcc
     6541 ggctataata agttcttgaa taaaataatt ttcccgacaa aacatgagta tttctttcga
     6601 aaataaaagt gcaggctaat tagagattat tctgtaatta actgcataat ttgtcacgtg
     6661 ccatagtttt acattccact acgtcatagt tcttaaaata ctaatctcct gaaaatagaa
     6721 gtaggtgaag aaagtttaat tatcagttct aaaatgacaa ttgatctttg gaatatgttc
     6781 tgaaactacc gatcattgaa cagatgctat ttgaatgata tagaattgta tatttgcaat
     6841 ttctgaaacg cgttcttaaa ggcacacaga ttaattcaaa agggtctggc cgcaaaaagg
     6901 tttatggtgg ccgattttga gttttgtgtg tgattgcttt ttcacaatca gtgttttcag
     6961 gattatgtga tgaactagat cttcaagttt cgttacattt catatgtttt cggaactcac
     7021 gaagtacata ttgggtattg tgctcaaaaa attcagcaat cagcttcgct ccgctgactt
     7081 tagaacccaa aaaaatagta tggccaaact gactgtgtta cgatcatttc aatttttcaa
     7141 tacatattta agatttctaa gagtaagaag gtcaaaaact gttctggaat acatatatat
     7201 ttttcaggtt acaattagtc aaaaagtgca ctgaaatata cgttttaatt tcacgaataa
     7261 cccaattagt tcaatgtatt tttggtcaac caacgttaaa gtttggcttc caaccaatta
     7321 tcatttctga tcaaccacaa tgttttttct ttatctgcaa gttaattttt tatttttatc
     7381 cagatgtttg gcatattttt caattcttca ctagcgccca cttcttgcac ttccggcgcc
     7441 ctgaatctaa tgcatctgtt gcaagaattg aaagaccaat caacacattg ttttcttcac
     7501 gagatactga agaaaatgaa taaaaacaga gaaaaagagc catgtgatta gtgacaactg
     7561 ttgctaacag ataccatagc ttggacttgg tacgtgatgg caacgtatgg gtcaacaaaa
     7621 atgattgcag agggggtgca aaacagtcaa gtcgagaaaa tatgaaaaac agaaaacaaa
     7681 gaacagaaaa atgggtttga gagtcagtat aatttataaa agaaaaattg tacatagaaa
     7741 ttaaccattt ttgtagaaga agttattttt caagcatcgt taaaaattat tcaaagcacc
     7801 ttatttcata tttaatttta aacatggtta aatgaacaac acggtgcgca atcaggaaaa
     7861 cttgaaatct gaaactgttg ttgtgatctt cttcgcaact gttcagatag cactagtgta
     7921 atgttaagag tgcgcgaata taatggaata taatggatca cacctcctgc catcaggtaa
     7981 acgtctctgt tatcacatat ttccaactat taaattttta ccttttacag ttttacattt
     8041 ttttgaaaaa agtaactttt tgtcttcaaa atccctgacg aaaatatcaa atattttaat
     8101 cgagactgca gaggaaccga ttgatgattt ggaaaatcca gctttacctg tgtaagaact
     8161 gaaaagtttc ataaccctag ggtattccca gttacattcc ccactggcta acaatagcac
     8221 ccagtttttc atcacctttc ttcaaatttc tcggcgattt gttaaaaaca aaatttgtgt
     8281 cccttctctg atatctctat gtctctaaac acaagttcat cggaaaacga aggagggtag
     8341 gtgttggttg ggctcccgaa gtgaaaatag aagagcaaga atagaatatt agagagagag
     8401 tgcagagagg gcgggatagc tcccgggatt ccgttttctt cttctttatc ttcaacgatg
     8461 atgtgtgtgc gtgttgtata gattctgttg ctcccccaca actcgctccg aaggctcaat
     8521 acaattcaat tgatattgga ggagagccta ccggagtggg aggataagaa gaaacataag
     8581 aagaagaaga agaagaagca tgcttctggt ttttgatgct atgaaaacgg cacaaaaaga
     8641 tgattgaggt cccttttcaa taccttctct catctttcaa atcccattga aacctaaaac
     8701 ttctcaccac gctttaccat tgttctccaa aaacttatag caatgtctat aactttttta
     8761 tctctgaaaa gcagtgttcc atttttcttt ttcctatttt atttcaattg tttctcacat
     8821 ttcgtttgga ttctttgctt gtcaaccagc ttcttcttcc acttttaccg tctaattttc
     8881 agggcaggga gccatcaaac ccacgaccac tagatccatc tagaaggccg gccatgagcg
     8941 atcagaagcc ggtaagtgaa tcttttcgaa tgttgctcaa catgattcac ggtatttcag
     9001 aatatggggc gcattgtcgc cagcgtcgtg gatgtgcagc agttgatgac aatgcagttt
     9061 gactcgctta tcaaaagcat ggactcgttg aaggtatgcg attcatgaaa aactcaattt
     9121 aatacttatt tttcacagat tgagcaccag accggcgtca cccagcttcg cgatgatatt
     9181 cgtcgatccg aggatcggtt tcagaagcag ttgtccgatt tgagcactaa tcacggaaaa
     9241 gagctcgaga gacttcatca aattatacac actcttcttg ctcgtgatgc gaatcctctc
     9301 ggctcaatga ttcctccaca gcagcttcag caacaacagc aaatgttgat tcttcagcga
     9361 caaatggaga tggctcatgt gcaggcagca caagctcaag ctcatgctca cgctcaagct
     9421 caagcccaag cacaggccca aagccaagtt atggctaatt tgctcaatgc tgcgaagcca
     9481 gctattcccg ttacacagcc actggtcgcc actacagcac aagcaaaatc gacagttcca
     9541 gcttctggag ttattgcacc aaaggtaaca aattttcggg ttttagttcc agcaattaaa
     9601 aattcaatat ttttcagaca tctccaccag aagttgtaat tccacctgct aagccaacat
     9661 tctctactcc aacgcctgca gttgttccaa agccagccac caccggattc tcattcggcg
     9721 ggaccaatcc agctacttca atcttcggta aaaagcctga aactgcttcc ccagtcgtgg
     9781 ttccagcagc taaagatgat gaagacgaag aacatgatga ggattacgag ccagagggcg
     9841 agttcaagcc agtcattcca ctgccagatc ttgtcgaggt gaaaactgga gaagaaggag
     9901 agcaaaccat gttctgcaat cgttcaaaac tctacattta tgccaacgag acgaaggaat
     9961 ggaaggagcg aggaaccggc gagctgaagg ttctctacaa caaggacaag aagtcgtgga
    10021 gagttgttat gcgacgagac caggtgacca tttcggagga gacactccct tcaaatattg
    10081 agaatggaac cgaagaattg tgttggaata ttcaggttat tccaattaca gcttttttca
    10141 catttttcgg cgttttcctc aacagaaaga tcttaattta aatttaaata ccttaattta
    10201 gcattaaaat aaattacagg ttctcaaagt ctgtgccaat ttcccaattc tcggctcgat
    10261 gaccattcaa caaatgaaat ccaacgagaa ggcctacaca tggttctgcg aggacttttc
    10321 cgaagatcag ccagctcacg tgaagctttc cgctcgtttc gctaacgtcg atatcgctgg
    10381 agagtttaag actttgttcg agaaggctgt cgctgaggca aaatcatcga gcaacgctgg
    10441 aaagaccatc gacaaggaaa tcaagccggc ggctgaggtg aagaaggaag tcaagcaaga
    10501 agttgtgatt ccatcaaaca acaaaccgga agagactgga tttggagatc agttcaagcc
    10561 aaaaccagga tcttgggaat gccctggatg ctatgttact tgcaaagctg acgagattga
    10621 atgtgcctgt tgtgggacat cgaaagacgg atctgtcaaa gagaaaaata tattctctaa
    10681 gtaaatattc tatttgagtt atcttaaaat taaaaattta aatttgcaga ccttcgatcc
    10741 tccagcctgc cccaggaact ccaaaagtga cattcggatt cggagcatct gctccagcca
    10801 aggaacctct tgctcaaaca tctcagttcg gtggatcact cagcggatct ccaagcacta
    10861 gcagtagcat tttcggtggt ggaacaccaa agggaacaag cgttttcggt ggaggtgctg
    10921 ctaatactcc aactttctca ttcaacaagc cagctgccgc tgtcaatgca actaccccat
    10981 cgttcaattt caacaatcct gcagcttcaa ctgcttctcc ggcaacctct acgactccag
    11041 gaaattcatt gttcggaggt ggactctcca agactgaatc tacagcttct tcaactacta
    11101 ctccgtcgtt tatgttcgcc aagaattctg aaagtgcctt cccaaagcca actttctcat
    11161 tcggaaagca acagactcca tcaacgactg caccagctaa acaagaagaa aataaacagt
    11221 ccgagacgcc aaaatccgtg ttcggtagtg gattcacgtc tggaggtgca acattcgctg
    11281 ccctttcagc caactctgcc aaatctggct ccattttcga cgcagcaaat gtcaagaagg
    11341 cccaagaaga acttgctgct cagaagaagg caagcatttt cgggagcaag aacaccacgt
    11401 tgaacacgac ttctgcaact tcacacgatg gtgatgagac gaatgaggat ggagatgggg
    11461 agtatgagcc agaagtcgag tttaagccag ttattcctct tccggatttg gttgaggtga
    11521 aaactggtga agaggacgag gaggtcatgt tctccgccag atgcaagctt tataagtact
    11581 attcggatct caaggagaat aaggaacgtg gactcggaga tattaaggtc agatttagga
    11641 ttttatatca aaatgagcca gcttggcatg ttcttttgat agttttcatt aaaatttagt
    11701 gataaaacgt agaaaataac ttatttcagt tgaaacagtt aagaaaagtg ctagttttgc
    11761 gcacttttca actgttttgc aagtattttc tatgtttttt cactaaattt taatgaaaac
    11821 tattaagaaa acaactgatt tttgactcat agaggctaaa tttgagaaaa ataatttatt
    11881 atttcagctc ctgaaaagca atgacaacaa atatcgaatc gtgatgcgtc gtgagcaagt
    11941 ccataagctc tgtgccaatt tccgtatcga aaagtcgatg aagctcagtc cgaagccaaa
    12001 cttgccaaat gtgctcacat ttatgtgcca ggtagcaatt tttaatttct ttttatataa
    12061 aaatgtacta taaattcctt atttttcagg acttctcaga agacgcctcc aacgctgatc
    12121 cagccatctt caccgccaaa ttcaaggacg aggcgaccgc cggagcattc aagacggctg
    12181 ttcaggatgc tcaatcgaag atgacctgca taatggtgag caagggcgag gaggataaca
    12241 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
    12301 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
    12361 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
    12421 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
    12481 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
    12541 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
    12601 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
    12661 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
    12721 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
    12781 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
    12841 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
    12901 gcatggacga gctgtacaag atggcttctt ctcttcgtca gatcctcgac tctcagaaga
    12961 tggagtggcg ttctaacgct ggaggatctg cggccgcaga ttacaaggat cacgatggcg
    13021 attacaagga tcacgatatc gattacaagg atgatgatga taagtaaccc gggctgcaga
    13081 ctactcttaa gtcctctgct gattatttaa attattttat tattgttatt cctccccacc
    13141 cagcactctg tgttagtatt ccccaccacc aattttattt atgaacctag ttttattcat
    13201 ccccatctgc tgtcatctat agtcctttaa gtgtagttta tatttgtctg gtaaggtttt
    13261 taaaaaaata atttccttat ttccccccca tctctctcaa aatcccattg ttctcataag
    13321 taccccccac acactatcat tcataatttt tgtctttaaa acttaactat attgttcaca
    13381 ttgtcccggc atcgattttt ccccactggt gacaatccaa taaattgtga atgcattgaa
    13441 gaatgagatt tagggcccgg tacccagctt ttgttccctt tagtgagggt taattgcgcg
    13501 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
    13561 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
    13621 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
    13681 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
    13741 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
    13801 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    13861 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    13921 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    13981 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    14041 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    14101 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    14161 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    14221 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    14281 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    14341 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    14401 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    14461 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    14521 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    14581 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    14641 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    14701 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    14761 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    14821 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    14881 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    14941 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    15001 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    15061 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    15121 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    15181 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    15241 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    15301 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    15361 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    15421 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    15481 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    15541 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    15601 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    15661 gccac
//
