LOCUS       sequence-62621-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Nevozhay D, Zal T, Balazsi G
  TITLE     Transferring a synthetic gene circuit from yeast to mammalian cells.
  JOURNAL   Nat Commun. 2013 Feb 5;4:1451. doi: 10.1038/ncomms2471.
  PUBMED    23385595
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013-02-5"; volume: "4:1451. doi"; pages: " 
            10.1038/ncomms2471"
COMMENT     Sequence Label: pDN-D2irG4kwh
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    214..232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    240..258
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(254..457)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(283..303)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        458..837
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 agaaagaaca atcaagggtc cccaaactca ccctgaagtt ctcaggatcg atccggagct
       61 ttttgcaaaa gcctaggcct ccaaaaaagc ctcttcacta cttctggaat agctcagagg
      121 ccctagagga tccccgggta ccaagcttaa gtttaaacgc tagagtccgg aggctggatc
      181 ggtcccggtg tcttctatgg aggtcaaaac agctctctat cactgatagg gagatctgat
      241 ctctatcact gatagggatg cttatataga cctcccaccg tacacgccta ccgcccattt
      301 gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa
      361 acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct
      421 atccacgccc attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata
      481 cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg
      541 cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat
      601 gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc
      661 ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg
      721 gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc
      781 tatgaactaa tgaccccgta attgattact attaataact agtcaataat caatgtcaac
      841 gcgtatatct ggcccgtaca tcgcgaagca gcgcaaaacg cctaacccta agcaga
//
