LOCUS       sequence-62623-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Nevozhay D, Zal T, Balazsi G
  TITLE     Transferring a synthetic gene circuit from yeast to mammalian cells.
  JOURNAL   Nat Commun. 2013 Feb 5;4:1451. doi: 10.1038/ncomms2471.
  PUBMED    23385595
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013-02-5"; volume: "4:1451. doi"; pages: " 
            10.1038/ncomms2471"
COMMENT     Sequence Label: pDN-D2irTNG4kwh
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      104..113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(821..842)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gatatacact gtttgagatg aggataaaat actctgagtc caaaccgggc ccctctgcta
       61 accatgttca tgccttcttc tttttcctac aggtcctgca ggcgccacca tgggttctag
      121 actggacaag agcaaagtca taaactctgc tctggaatta ctcaatgaag tcggtatcga
      181 aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg
      241 gcacgtgaag aacaagcggg ccctgctcga tgccctggca atcgagatgc tggacaggca
      301 tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc
      361 caagtcattc cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac
      421 ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca
      481 aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg
      541 ctgcgtattg gaggatcagg agcatcaagt agcaaaagag gaaagagaga cacctaccac
      601 cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccatc agggagccga
      661 acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg
      721 cgaaagcggc gggccaaaaa agaagagaaa gggtgacggt gctggtttaa ttaacatggt
      781 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      841 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctac
//
