LOCUS       sequence-62626-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Nevozhay D, Adams RM, Murphy KF, Josic K, Balazsi G
  TITLE     Negative autoregulation linearizes the dose-response and suppresses 
            the heterogeneity of gene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 
            10.1073/pnas.0809901106. Epub 2009 Mar 11.
  PUBMED    19279212
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0809901106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-03-31- 31"; volume: "106"; issue: "13"; pages: "5123-8"
COMMENT     Sequence Label: pDN-G1Gh
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(43..484)
                     /label=TPGI promoter
                     /note="S. cerevisiae GAL1 promoter modified to contain two 
                     copies of the tet operator (Ellis et al., 2009)"
     primer_bind     complement(49..72)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    120..138
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    141..159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    367..484
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     protein_bind    615..631
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 attcttcacc tttagacatt ttaataggat ccggggtttt ttctccttga cgttaaagta
       61 tagaggtata ttaacaattt tttgttgata cttttattac atttgaataa gaagtaatat
      121 ctctatcact gatagggaga tctctatcac tgatagggac agtttttgca tttatatatc
      181 tgttaataga tcaaaaatca tcgcttcgct gattaattac cccagaaata aggctaaaaa
      241 actaatcgca ttatcatcct atggttgtta atttgattcg ttcatttgaa ggtttgtggg
      301 gccaggttac tgccaatttt tcctcttcat aaccataaaa gctagtattg tagaatcttt
      361 attgttcgga gcagtgcggc gcgaggcaca tctgcgtttc aggaacgcga ccggtgaaga
      421 cgaggacgca cggaggagag tcttccttcg gagggctgtc acccgctcgg cggcttctaa
      481 tccgtacttc aatatagcaa tgagcagtta agcgtattac tgaaagttcc aaagagaagg
      541 tttttttagg ctaagataat ggggctcttt acatttccac aacatataag taagattaga
      601 tatgttgtgt ggaattgtga gcggataaca atttcacaca tgtaatatga ttattaaact
      661 tctttgcgtc catccaaaaa aaaagtaaga atttttgaaa attcgaattc aaccctcact
      721 aaagggcggc cgcactagtg agaatgcatt atatgcactc agcgctgtgg ggcattttac
      781 tttaggttgc gtattggaag atcaagagca tcaagtcgct aaagaagaaa gggaaacacc
      841 tactactgat agtatgccgc cattattacg acaagctatc gaattatttg atcaccaagg
      901 tgcagagcca gccttcttat tcggccttga attg
//
