LOCUS       sequence-62629-e         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Nevozhay D, Adams RM, Murphy KF, Josic K, Balazsi G
  TITLE     Negative autoregulation linearizes the dose-response and suppresses 
            the heterogeneity of gene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 
            10.1073/pnas.0809901106. Epub 2009 Mar 11.
  PUBMED    19279212
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0809901106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-03-31- 31"; volume: "106"; issue: "13"; pages: "5123-8"
COMMENT     Sequence Label: pDN-G1TGt
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(49..669)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     577..596
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(699..722)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 gaataattct tcacctttag acatgttaat taaaccagca ccgtcaccag acccactttc
       61 acatttaagt tgtttttcta atccgcatat gatcaattca aggccgaata agaaggctgg
      121 ctctgcacct tggtgatcaa ataattcgat agcttgtcgt aataatggcg gcatactatc
      181 agtagtaggt gtttcccttt cttctttagc gacttgatgc tcttgatctt ccaatacgca
      241 acctaaagta aaatgcccca cagcgctgag tgcatataat gcattctcta gtgaaaaacc
      301 ttgttggcat aaaaaggcta attgattttc gagagtttca tactgttttt ctgtaggccg
      361 tgtacctaaa tgtacttttg ctccatcgcg atgacttagt aaagcacatc taaaactttt
      421 agcgttatta cgtaaaaaat cttgccagct ttccccttct aaagggcaaa agtgagtatg
      481 gtgcctatct aacatctcaa tggctaaggc gtcgagcaaa gcccgcttat tttttacatg
      541 ccaatacaat gtaggctgct ctacacctag cttctgggcg agtttacggg ttgttaaacc
      601 ttcgattccg acctcattaa gcagctctaa tgcgctgtta atcactttac ttttatctaa
      661 tctagacatt ttaataggat ccggggtttt ttctccttga cgttaaagta tagaggtata
      721 ttaacaattt tttgttgata cttttattac at
//
