LOCUS       sequence-62632-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Nevozhay D, Adams RM, Murphy KF, Josic K, Balazsi G
  TITLE     Negative autoregulation linearizes the dose-response and suppresses 
            the heterogeneity of gene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 
            10.1073/pnas.0809901106. Epub 2009 Mar 11.
  PUBMED    19279212
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0809901106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-03-31- 31"; volume: "106"; issue: "13"; pages: "5123-8"
COMMENT     Sequence Label: pDN-G1Tt
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(77..518)
                     /label=TPGI promoter
                     /note="S. cerevisiae GAL1 promoter modified to contain two 
                     copies of the tet operator (Ellis et al., 2009)"
     primer_bind     complement(83..106)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    154..172
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    175..193
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    401..518
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     protein_bind    649..665
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ttaagcagct ctaatgcgct gttaatcact ttacttttat ctaatctaga cattttaata
       61 ggatccgggg ttttttctcc ttgacgttaa agtatagagg tatattaaca attttttgtt
      121 gatactttta ttacatttga ataagaagta atatctctat cactgatagg gagatctcta
      181 tcactgatag ggacagtttt tgcatttata tatctgttaa tagatcaaaa atcatcgctt
      241 cgctgattaa ttaccccaga aataaggcta aaaaactaat cgcattatca tcctatggtt
      301 gttaatttga ttcgttcatt tgaaggtttg tggggccagg ttactgccaa tttttcctct
      361 tcataaccat aaaagctagt attgtagaat ctttattgtt cggagcagtg cggcgcgagg
      421 cacatctgcg tttcaggaac gcgaccggtg aagacgagga cgcacggagg agagtcttcc
      481 ttcggagggc tgtcacccgc tcggcggctt ctaatccgta cttcaatata gcaatgagca
      541 gttaagcgta ttactgaaag ttccaaagag aaggtttttt taggctaaga taatggggct
      601 ctttacattt ccacaacata taagtaagat tagatatgtt gtgtggaatt gtgagcggat
      661 aacaatttca cacatgtaat atgattatta aacttctttg cgtccatcca aaaaaaaagt
      721 aagaattttt gaaaattcga attcaaccct cactaaaggg cggccgcact agtgagaatg
      781 cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag
      841 agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccc
//
