LOCUS       sequence-62634-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Nevozhay D, Adams RM, Murphy KF, Josic K, Balazsi G
  TITLE     Negative autoregulation linearizes the dose-response and suppresses 
            the heterogeneity of gene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 
            10.1073/pnas.0809901106. Epub 2009 Mar 11.
  PUBMED    19279212
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0809901106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-03-31- 31"; volume: "106"; issue: "13"; pages: "5123-8"
COMMENT     Sequence Label: pDN-G1Tt
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..639
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(89..108)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      672..861
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
ORIGIN
        1 ccggatccta ttaaaatgtc tagattagat aaaagtaaag tgattaacag cgcattagag
       61 ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt
      121 gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta
      181 gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg
      241 caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat
      301 ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat
      361 caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc
      421 gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa
      481 gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa
      541 ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc
      601 ggattagaaa aacaacttaa atgtgaaagt gggtcttaac tcgagtaagc ttggtaccgc
      661 ggctagctaa gatccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc
      721 cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt
      781 cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag
      841 aaggttttgg gacgctcgaa gatccagctg cattaatgaa tcggccaacg cgcggggaga
      901 ggcggtttgc gtattg
//
