LOCUS       sequence-62639-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Nevozhay D, Adams RM, Murphy KF, Josic K, Balazsi G
  TITLE     Negative autoregulation linearizes the dose-response and suppresses 
            the heterogeneity of gene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 
            10.1073/pnas.0809901106. Epub 2009 Mar 11.
  PUBMED    19279212
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0809901106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-03-31- 31"; volume: "106"; issue: "13"; pages: "5123-8"
COMMENT     Sequence Label: pDN-T1TCbt
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(183..803)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     711..730
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 gtttgggttc cttcataagg ccttccttct ccttcacctt ctatttcaaa ttcatgtcca
       61 tttacagaac cttccatgtg aactttgaat ctcatgaact ccttaatgat tgccatgtta
      121 ttttcttctc ctttactaac catgccgcta gttgccatgt taattaaacc agcaccgtca
      181 ccagacccac tttcacattt aagttgtttt tctaatccgc atatgatcaa ttcaaggccg
      241 aataagaagg ctggctctgc accttggtga tcaaataatt cgatagcttg tcgtaataat
      301 ggcggcatac tatcagtagt aggtgtttcc ctttcttctt tagcgacttg atgctcttga
      361 tcttccaata cgcaacctaa agtaaaatgc cccacagcgc tgagtgcata taatgcattc
      421 tctagtgaaa aaccttgttg gcataaaaag gctaattgat tttcgagagt ttcatactgt
      481 ttttctgtag gccgtgtacc taaatgtact tttgctccat cgcgatgact tagtaaagca
      541 catctaaaac ttttagcgtt attacgtaaa aaatcttgcc agctttcccc ttctaaaggg
      601 caaaagtgag tatggtgcct atctaacatc tcaatggcta aggcgtcgag caaagcccgc
      661 ttatttttta catgccaata caatgtaggc tgctctacac ctagcttctg ggcgagttta
      721 cgggttgtta aaccttcgat tccgacctca ttaagcagct ctaatgcgct gttaatcact
      781 ttacttttat ctaatctaga cattttaata ggatccgggg tttt
//
