LOCUS       sequence-62645-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Nevozhay D, Adams RM, Murphy KF, Josic K, Balazsi G
  TITLE     Negative autoregulation linearizes the dose-response and suppresses 
            the heterogeneity of gene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 
            10.1073/pnas.0809901106. Epub 2009 Mar 11.
  PUBMED    19279212
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0809901106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-03-31- 31"; volume: "106"; issue: "13"; pages: "5123-8"
COMMENT     Sequence Label: pDN-G1GZmbh
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(42..483)
                     /label=TPGI promoter
                     /note="S. cerevisiae GAL1 promoter modified to contain two 
                     copies of the tet operator (Ellis et al., 2009)"
     primer_bind     complement(48..71)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    119..137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    140..158
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    366..483
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     protein_bind    614..630
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ttcttcacct ttagacattt taataggatc cggggttttt tctccttgac gttaaagtat
       61 agaggtatat taacaatttt ttgttgatac ttttattaca tttgaataag aagtaatatc
      121 tctatcactg atagggagat ctctatcact gatagggaca gtttttgcat ttatatatct
      181 gttaatagat caaaaatcat cgcttcgctg attaattacc ccagaaataa ggctaaaaaa
      241 ctaatcgcat tatcatccta tggttgttaa tttgattcgt tcatttgaag gtttgtgggg
      301 ccaggttact gccaattttt cctcttcata accataaaag ctagtattgt agaatcttta
      361 ttgttcggag cagtgcggcg cgaggcacat ctgcgtttca ggaacgcgac cggtgaagac
      421 gaggacgcac ggaggagagt cttccttcgg agggctgtca cccgctcggc ggcttctaat
      481 ccgtacttca atatagcaat gagcagttaa gcgtattact gaaagttcca aagagaaggt
      541 ttttttaggc taagataatg gggctcttta catttccaca acatataagt aagattagat
      601 atgttgtgtg gaattgtgag cggataacaa tttcacacat gtaatatgat tattaaactt
      661 ctttgcgtcc atccaaaaaa aaagtaagaa tttttgaaaa ttcgaattca accctcacta
      721 aagggcggcc gcactagtta agagctctta attaacaatt cttcgccaga ggtttggtca
      781 agtctccaat caaggttgtc ggcttgtcta ccttgccaga aatttacgaa aagatggaaa
      841 agggtcaaat cgttggtaga tacgttgttg acacttctaa
//
