LOCUS       sequence-62647-e       12471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12471)
  AUTHORS   Steiner FA, Talbert PB, Kasinathan S, Deal RB, Henikoff S
  TITLE     Cell-type-specific nuclei purification from whole animals for 
            genome-wide expression and chromatin profiling.
  JOURNAL   Genome Res. 2012 Apr;22(4):766-77. doi: 10.1101/gr.131748.111. Epub 
            2012 Jan 4.
  PUBMED    22219512
REFERENCE   2  (bases 1 to 12471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.131748.111"; journalName: "Genome Res"; date: "2012-04";
            volume: "22"; issue: "4"; pages: "766-77"
COMMENT     Sequence Label: pSO221 Phis-72 BirA GFP
FEATURES             Location/Qualifiers
     source          1..12471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4424..4435
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             7420..8379
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             7420..8379
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(8425..8453)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     9201..9221
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(10283..10303)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10283..10301)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10322..10338)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10322..10338)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10335..10357)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10346..10362
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10370..10376,10377..10394,10395..10400))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10415..10436
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10553..10570)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10724..11312)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10804..10823)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11483..12274,12275..12343))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12106..12125
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12344..12448)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgctcta gagaatttta acaatacttc aaatttgtta agccgaacat
      721 aacaaaaaat gaataaaaaa cggaaagtga ttgacccgta tttgaagaag aagacaaatg
      781 agtgggggcg acaatggggg aaaaagagac gatgaaagtg aagtggaatt aaatgtcgga
      841 aaattttgtg gaaaaagaat aatgaaatat acatataata tcagtatctt cagcttttcc
      901 tcctgcttca caatctcggc ggcagattta tgtaaatgat atctccattc aaatggtatt
      961 caaagaagaa gaagaagaag gcaattgctg ataatgagat aaaaggaggc ttctcctact
     1021 gataacaatt ggtcgggaat tcatgattag tggaagaaaa gggtggtgtt ttggatgaag
     1081 aagatgttgg gaatgccaat gatcggattt ttgatgcaag cagttatctt ttgcaacatg
     1141 attagtcatt taaatcaggg ttggcgacaa acaatttttt tccggcaaat cggcaaatcc
     1201 gttagttgcc ggaattggta acttccggca aatcgacaaa tggtcggaat tggaatgttc
     1261 ggcaaatcgg caaatcggca atttgtcgga attgaaattt ccgacaaagc ggcaaatcgg
     1321 cagtttttcg gaattgacat ttttggcaaa tcggcaaatc ggttagatgc cggaactgaa
     1381 aacttccggc aaagcggcaa atcgctgact ttccaatttg attttgttga gaagttttat
     1441 ctaaagttaa atttctagct aatcggcaag tccgttaatt gccggaattg gaaatttccg
     1501 gcaaatcggc aaagcggcac attgtcggaa ttgaaatttc tagctaatcg gcaaatcggt
     1561 tagttgccgg aattggaaac ttccggcaaa gcgacaaatc acagattttc caaatttaat
     1621 ttttatgata attttttgta aaagggccga attatttttg aataattcaa gtttttttta
     1681 attttatggt tttttccaac attcaaataa aacatatcaa ttgagaacat tatatttgaa
     1741 aaatcattta ttgggatttg tattgaaaag ctgaaaaatg agagttttcg gaatgcattt
     1801 tgtgagaaat atagtgaaaa attaggaaat tttaaattta gcggtaggaa aatcacatga
     1861 tttctataaa ttgcgagaaa aattattagt tttattccat cggcgggaaa ttatgcatca
     1921 tacgagtagt cggccttatt gtgcattacg agcttattct ccacaaaata gaagctatcg
     1981 gagcgggtct cgtttggatt attgatcatg tcgtccactg aaaatataca gttttttttt
     2041 taattttaca acaaaaaaac atattttttt tttgcaaatt tcattttact ttccaattag
     2101 tcagtttttt tccgcgataa tttttaattt catgggctag ttttggcaag aattcttctc
     2161 ttcgatgcac catgagcact taaaaaaaat ttgcgaactt ttttatcggt ttaatgtagt
     2221 ttttaacaaa atttctcaaa tgttcaatac atttccagaa attttgaatt tcccgtcaaa
     2281 taattttctc agaaaatttg aattctcgcg gctaattttt ttctcagaaa atttgaatta
     2341 cccgccaaaa acttctcaga gaaaactcga atttcctcca agttttcaca ttttgaacta
     2401 ctgtagggca gctttttgtc aatatttatt ttatttttaa aaacctagaa aattcgctct
     2461 attgacaaaa tgagctcaaa taatttttcc cgcttcaaac tcactgagtt gttgtgaaag
     2521 ttgtggaaat tcatagatat gttcacagtt gtttctcgaa tttggcatac agaatccaaa
     2581 ttcaaaatca aagcatttca ataaacgatc cttgaaaaag tgccgttcaa tcattcgaaa
     2641 atgtgtgatc ggcacatcgc cgaccttgaa ctcgaccgtc gcgccgaccg tctttaattt
     2701 cagaaaattc ggcgcaaatc gatatcggac atatcttgcc gattcggctt gcgcctgcag
     2761 attctcttcc gtctcatttt ccggttttgc aatttcgaag agcacgtgtt ccgtgtcgag
     2821 gtcacggatt tggaatttgg tgaactcaat gttatagacg tttgccgatg gggagcatag
     2881 gaatcctgaa aatttgatat gtttgtccac gtggagtaca cggcgcgttt atcaatagag
     2941 caaatattca cttaccttga gtgattcctg gaagagctag cacatcattt ggtgtaattt
     3001 gatttttcgc gagaagctcc gcctcggtgg ttatagcctg ttcggttacc ggtgggggtc
     3061 ggggcatctg aaaaaaaaaa gagttttctg tattctgcca aggaaacagc aaattatgtt
     3121 cggcaaaaat agacaaaaag taattgagaa aattgaccgg tgctggcatg caatttttgt
     3181 acatagatgt gaatttagcc tatttcaatt tattattttt gtgtgaattt tttttttaat
     3241 attcaaacag tgcacgcttt tttgggaacc aggtgttggc ggcagttgcc gttcggcaaa
     3301 ttgcaggttt gccgatttga cgaaactttg tatttccggc aattttccgt tttccggaaa
     3361 tttttagaga aatattttat aagacggaaa cacttaaaac tgtgcctttt tgaaattttt
     3421 tttcggcaaa tcaacaattt gccagaaact ttaaattccg gcaatttgcc aatttgccga
     3481 tttgtcggaa aaaatcattt gccgccaacc ccacgtggtg ccaggctgtc ccatcacggt
     3541 ttgatataca aaaaatgcgg gaattctttg tccaaaaaga tgtgacgtca gcacgttcat
     3601 aaaaggggtg tgcggcaaat ctcaaaattt gcccagctcg gcaaacggca aatttgccga
     3661 acacccctgg ttcataacca tgtgaaatca gttgagaact ctgcgtctct tctcccgcat
     3721 tttttgtaga tcaacgtaga tcaaaccgaa atggaacact ctgacaccac gtgccacccc
     3781 tgattgaacc accgaaaggc acatatacaa atgttcaaaa atgagtggca aaaactagcg
     3841 aaatttgact gcacaaaaaa ataattttta aaattttctt ttaaaatcta ctgtgttatt
     3901 cggtcatttt agcgctataa aagtagattc taatagtaag tgcaaaattg caaattaaat
     3961 ttatttggag caactctaag tctctctaaa atccgcgttt tttgagaaaa gttcaaacaa
     4021 tttcaacgta gaagaaaaga aagcaagcat atcgatatat gaaattggat atcaggagtt
     4081 agtatactgc gtgcataaac acgctctctc tctcatttct ctgcgtcttc ctcacctgag
     4141 acgggaaggt tgccgagccg ggtgcgatcg attgttgttg ttgctctgcc ttcatatatg
     4201 ctgttgtagc tgaaaatttt ggggattatg ggaattttgg agagtgctct ggagattaga
     4261 ggtggtgtgg ctgcggaaaa aattcggcca ccagtatttt ctaggccgtg ggggataggg
     4321 ttattcttta agcaaatttc aggtttccaa gtttttattg taaattttga gaattaaaat
     4381 aaaaattata aaagttctgg aaaatatttc cgtatgaatt tcctgggaac ttcaaatttt
     4441 tttgtacaaa gtgcaaaaat atatttaatt taaattggtt taaaaaattc ggccaccagt
     4501 tttttctatg ccgcgaggta tagtgttagt gtgtttttga caactttcaa ttttttgaga
     4561 aaattttacc aaaaatattc caagttttca tcataaattt cctgggaact taataatatt
     4621 ttttgagcac aatttggtag aaaatatttt ttcaaatttt caaattttgt aaaattggtg
     4681 aaatattttg aattcacttt caattttatt tattcaagga atattttgaa aaaaaatgtg
     4741 tttttaattc acaaataatt actaaaaata actaaaaaat aacactgttt tatgggattt
     4801 acagtttttg tttaaaatcc tgaaatattt tttttactta aacattaata atttttttat
     4861 gagtaaagaa atatgcaatt caaaaatatt tttaaaatat ttgaacgcat ccaattaatt
     4921 gattaaattt atgatgtttg cgtttttttt tgagtttttg aaaagatatg ttcccaaaat
     4981 ttttggaaat gtttgtaaaa atttttagat tttttactcc ggaaaccctt tttatggaaa
     5041 actttcaaac ccctctaatg taccaaaaga gcaaaaaatc aatttttgca ctgaataacc
     5101 ttttaactaa ctaaaatgaa aaagaaattc caacaaaaaa taaatgagtt ccgtgtgctc
     5161 ttgctcgcag agcacatcca aaaattaatg ggggtcgtcc gtaatgattg acagagatgg
     5221 aaaatggcga aaaagggctg aagaagatga aaaaagaaga cgtctaggaa aagagaagcg
     5281 agagagtgtg gggaacgctg agggcgcaga gagaggaacg gacaaaatga cagatgatga
     5341 gcaaagagaa agagagagag ggagagagaa agagagggag agagggatga atttgtgatg
     5401 aaatagacag tgagagaaaa tgagaggaaa agatggtaat tcaatagaat cttagagcaa
     5461 tttgaagttt gagtcattac ttttaaagtg ggcatcttta aaggtagaat catttaggat
     5521 tttatctaaa taaatctata ataaaccaat atgactgaat gtcattttgg tacactgcca
     5581 aaattcaaaa aaaaattgga gaatttgaga aattttgagc tttgctacca gtttgaacct
     5641 ctggaacagg ggtgggcggc aattgccttc cggcaaattt cgccaaattg ccggtttgcc
     5701 gatttgtcgg aaattttaat tttcggcaaa aatgcggatt tgctctttta cggatatcaa
     5761 tttgccggaa gtttttagag ggatttagta taattcggaa acacttgaaa ctgtgccttt
     5821 ttgaaattgt ttccccattt tctttagaca ttttcataga attcgcttac ttttcaaaat
     5881 agatgtagga acattcttag gatgcctaca gttttgccga ttaaaattga aattctgaaa
     5941 tttccaaaaa aaaaaatttt aaaaccacaa tttgccattt tcgtgtcaaa aactttcggc
     6001 aattaccgtt tttccggcaa attcagcaaa ttggcaattt gccggttccc caatttgccg
     6061 gaaattttca gttctggcaa tttgccaatg gtttttgaaa cattttacag taatatataa
     6121 ggatttcatg ggtaactata ggactatagg actataggag tggtttaaaa aactgaattt
     6181 tgagagactt gacacatttt ccaaaacatt ttttttaatg ttacgtttga cttaaagcaa
     6241 tgataagata tgttaagatt ttgaagaatt tagatatgag gttatagaat actttgcatt
     6301 agtgatgtat ttatttgaaa gaacattgat ggggagaatt tttcaaacag aaaaaaaact
     6361 ataaaattct acttctttta ctgaagcttg tcgaccctag ggggcccaaa cgttatagtg
     6421 tggacaccaa tttggcttcg tacttcttac tcgttcccgc cccgccccga caccattcga
     6481 atatatattg tcagttgttc tgtttgtcgt cgtgatgaga ggtgcgtgcg tgtgcgcgcg
     6541 cgctctccca acttttcgga ccattttgcg ggctttatac tgcaatttgg ctcatttctt
     6601 ctcctttttt ttccacaggc ttattttgta ctctcgagtt gacagccttt ttcggtatag
     6661 gttggaagta gtccattaaa gtagatgttt cttaagaatt tatcggctta gcatcgattt
     6721 cgacctagtt agatgccacg tttatgcgcg aagcggccgc gtcgcggcga gaatttcagt
     6781 gcggttttac gggattatcc attaaaatca ggaaaatcag cagaaaactt cttaacaaaa
     6841 ctaatttttt tagaaaagaa tttagaaata cgtagtcttg aataattcag caactttttc
     6901 aaattttatg agaatttcgc aatttcctac acctcctatt tcatcatcac ttccgaaatc
     6961 ctacgttatc gatcgaacac gcgacgcgac cgcagcgcac ctactaagcg cgcgcatttc
     7021 gaccgtgacg cgaccgcaac gcggtggtgt cagggtgcgc gcgctttttc aatcatatgt
     7081 ggtgcggtcg attccttcat tcatccttct gtatgcagct gctcatcagt ttattgagca
     7141 tctgctgtac caattcgtac tttaaattaa atattaagag tacattttct gtgtgctctg
     7201 tatataaatg tctagaataa actgcaaaca agaattcttg gctccatctt tccctgaatc
     7261 aatatcgatc gatagtgaca cgcgacgcga ccgcaacgcg tagggccatt tcatattgat
     7321 tcttcttcta ctcttccttc ttctccttct gctctcacat ttcctcacta ttccaaccat
     7381 tctcaattct caatttccag aacaacaatg gctggatcca aggataacac cgtgccactg
     7441 aaattgattg ccctgttagc gaacggtgaa tttcactctg gcgagcagtt gggtgaaacg
     7501 ctgggaatga gccgggcggc tattaataaa cacattcaga cactgcgtga ctggggcgtt
     7561 gatgtcttta ccgttccggg taaaggatac agcctgcctg agcctatcca gttacttaat
     7621 gctaaacaga tattgggtca gctggatggc ggtagtgtag ccgtgctgcc agtgattgac
     7681 tccacgaatc agtaccttct tgatcgtatc ggagagctta aatcgggcga tgcttgcatt
     7741 gcagaatacc agcaggctgg ccgtggtcgc cggggtcgga aatggttttc gccttttggc
     7801 gcaaacttat atttgtcgat gttctggcgt ctggaacaag gcccggcggc ggcgattggt
     7861 ttaagtctgg ttatcggtat cgtgatggcg gaagtattac gcaagctggg tgcagataaa
     7921 gttcgtgtta aatggcctaa tgacctctat ctgcaggatc gcaagctggc aggcattctg
     7981 gtggagctga ctggcaaaac tggcgatgcg gcgcaaatag tcattggagc cgggatcaac
     8041 atggcaatgc gccgtgttga agagagtgtc gttaatcagg ggtggatcac gctgcaggaa
     8101 gcggggatca atctcgatcg taatacgttg gcggccatgc taatacgtga attacgtgct
     8161 gcgttggaac tcttcgaaca agaaggattg gcaccttatc tgtcgcgctg ggaaaagctg
     8221 gataatttta ttaatcgccc agtgaaactt atcattggtg ataaagaaat atttggcatt
     8281 tcacgcggaa tagacaaaca gggggcttta ttacttgagc aggatggaat aataaaaccc
     8341 tggatgggcg gtgaaatatc cctgcgtagt gcagaaaaat cgagaccggt agctactagt
     8401 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
     8461 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
     8521 acccttaaat ttatttgcac tactggaaaa ctacctgttc catgggtaag tttaaacata
     8581 tatatactaa ctaaccctga ttatttaaat tttcagccaa cacttgtcac tactttctgt
     8641 tatggtgttc aatgcttctc gagataccca gatcatatga aacggcatga ctttttcaag
     8701 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac
     8761 tacaagacac gtaagtttaa acagttcggt actaactaac catacatatt taaattttca
     8821 ggtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
     8881 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
     8941 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttgtaag tttaaacatg
     9001 attttactaa ctaactaatc tgatttaaat tttcagaact tcaaaattag acacaacatt
     9061 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
     9121 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
     9181 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
     9241 ggcatggatg aactatacaa ataagctcca tcaccaattc tcgaagcact tttaatattc
     9301 atttaatcca acttgtacca taatcctcgt atttttccat cccgaaaatt gatttttgcg
     9361 atcgaaccgc cgatctcatc accacttttc tctccgttct ttatgtctta ttttattcgt
     9421 aaattgctga atttcatgtt gaactccaac aaaaaaaaat aaagtttcgt gtgcttcagt
     9481 agtctagcaa catcatatga acgcctagca actatattcc acgccgccgc agttttgaat
     9541 tgaattcact aaattcatgc tctgaattca caaaaaaaat gtttcttcga gtcattggaa
     9601 atagtaaaaa acgccagcga ctatttatca gtaagtttta aaagggtctc gtcgcgaaat
     9661 tcacattaaa aatattaatt ttctgtatgt acctttaaaa ttactgtaga aaatctatca
     9721 gatgggtctt ggcgcgcaaa acgcaaaaaa taaattgatt tcattgcatg ctttttccgc
     9781 gccgagaccc atctaaccta aatttttcaa aaaggtgtgc gcctttaaag attactgtag
     9841 tttcgaactt gtgtagttgc ggagttttca tcgatttttt tgtaaataaa tagttttatt
     9901 ttagtttttg aagaaataaa tattttttca acgaacaact atatgaaaaa tcgataaaaa
     9961 tcccgcaatt ttttggaaaa ttctgtgatg gataatacca aattcagatg tttccttctt
    10021 ttttctcatc attgggcttc ttgttctcgg tgtcttcaca atcaaaaaga acacctatat
    10081 caccttcgat tcatgggatc tagcacaaga gattgctcag aacgcgacgg atgcagcgat
    10141 ggaaaactct tctgtaagtt tttcacggaa ttctggcctc cctcataaat cgaaatggca
    10201 gagtttgccg aactaggcca ttttgagtcc gcgagatttt gtgtaggttt acggcgcgtt
    10261 gcgtggtacc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
    10321 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
    10381 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
    10441 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
    10501 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
    10561 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    10621 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    10681 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    10741 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    10801 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    10861 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    10921 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    10981 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    11041 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    11101 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    11161 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    11221 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    11281 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    11341 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    11401 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    11461 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    11521 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    11581 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    11641 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    11701 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    11761 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    11821 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    11881 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    11941 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    12001 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    12061 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    12121 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    12181 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    12241 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    12301 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    12361 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    12421 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca c
//
