LOCUS       sequence-62649-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Nevozhay D, Adams RM, Murphy KF, Josic K, Balazsi G
  TITLE     Negative autoregulation linearizes the dose-response and suppresses 
            the heterogeneity of gene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 
            10.1073/pnas.0809901106. Epub 2009 Mar 11.
  PUBMED    19279212
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0809901106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-03-31- 31"; volume: "106"; issue: "13"; pages: "5123-8"
COMMENT     Sequence Label: pDN-T1GZmbh
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(50..73)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    94..112
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    121..139
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    142..160
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    368..485
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     protein_bind    616..632
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 aattcttcac ctttagacat tttaatagga tccggggttt tttctccttg acgttaaagt
       61 atagaggtat attaacaatt ttttgttgat acttctctat cactgatagg gaaagtaata
      121 tctctatcac tgatagggag atctctatca ctgataggga cagtttttgc atttatatat
      181 ctgttaatag atcaaaaatc atcgcttcgc tgattaatta ccccagaaat aaggctaaaa
      241 aactaatcgc attatcatcc tatggttgtt aatttgattc gttcatttga aggtttgtgg
      301 ggccaggtta ctgccaattt ttcctcttca taaccataaa agctagtatt gtagaatctt
      361 tattgttcgg agcagtgcgg cgcgaggcac atctgcgttt caggaacgcg accggtgaag
      421 acgaggacgc acggaggaga gtcttccttc ggagggctgt cacccgctcg gcggcttcta
      481 atccgtactt caatatagca atgagcagtt aagcgtatta ctgaaagttc caaagagaag
      541 gtttttttag gctaagataa tggggctctt tacatttcca caacatataa gtaagattag
      601 atatgttgtg tggaattgtg agcggataac aatttcacac atgtaatatg attattaaac
      661 ttctttgcgt ccatccaaaa aaaaagtaag aatttttgaa aattcgaatt caaccctcac
      721 taaagggcgg ccgcactagt taagagctct taattaacaa ttcttcgcca gaggtttggt
      781 caagtctcca atcaaggttg tcggcttgtc taccttgcca gaaatttacg aaaagatgga
      841 aaagggtcaa atcgttggta gatacgttgt tgacacttct aaataagcga atttcttatg
      901 atttatgatt t
//
