LOCUS       sequence-62651-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Nevozhay D, Adams RM, Murphy KF, Josic K, Balazsi G
  TITLE     Negative autoregulation linearizes the dose-response and suppresses 
            the heterogeneity of gene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 
            10.1073/pnas.0809901106. Epub 2009 Mar 11.
  PUBMED    19279212
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0809901106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-03-31- 31"; volume: "106"; issue: "13"; pages: "5123-8"
COMMENT     Sequence Label: pDN-T1GZmbh
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(138..851)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 aagtcgtcct ccacgaagtc ccgggagaac ccgagccggt cggtccagaa ctcgaccgct
       61 ccggcgacgt cgcgcgcggt gagcaccgga acggcactgg tcaacttggc catgttaatt
      121 aaaccagcac cgtcaccttt gtacaattca tccataccat gggtaatacc agcagcagta
      181 acaaattcta acaagaccat gtggtctctc ttttcgtttg gatctttgga taaggcagat
      241 tgagtggata agtaatggtt gtctggtaac aagactggac catcaccaat tggagtattt
      301 tgttgataat ggtcagctaa ttgaacagaa ccatcttcaa tgttgtgtct aattttgaag
      361 ttaactttga taccattctt ttgtttgtca gccatgatgt aaacattgtg agagttatag
      421 ttgtattcca atttgtgacc taaaatgtta ccatcttctt taaaatcaat accttttaat
      481 tcgattctat taactaaggt atcaccttca aacttgactt cagctctggt cttgtagtta
      541 ccgtcatctt tgaaaaaaat agttctttct tgaacataac cttctggcat ggcagacttg
      601 aaaaagtcat gttgtttcat atgatctggg tatctcgcaa aacattgaac accataaccg
      661 aaagtagtga ctaaggttgg ccatggaact ggcaatttac cagtagtaca aataaatttt
      721 aaggtcaatt taccgtaagt agcatcacct tcaccttcac cggagacaga aaatttgtga
      781 ccattaacat caccatctaa ttcaaccaaa attgggacaa caccagtgaa taattcttca
      841 cctttagaca ttttaatagg atccggggtt ttttctcctt gacgttaa
//
