LOCUS       sequence-62673-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Reed B, Blazeck J, Alper H
  TITLE     Evolution of an alkane-inducible biosensor for increased 
            responsiveness to short-chain alkanes.
  JOURNAL   J Biotechnol. 2012 Apr 15;158(3):75-9. doi: 
            10.1016/j.jbiotec.2012.01.028. Epub 2012 Feb 2.
  PUBMED    22326628
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jbiotec.2012.01.028"; journalName: "J Biotechnol"; date: 
            "2012-04-15- 15"; volume: "158"; issue: "3"; pages: "75-9"
COMMENT     Sequence Label: pACYC-PLlambda-alkS(WT)
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(100..118,119..124,125..141,142..147,148..173)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    100..118
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             183..194
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 atatttctag acaattccga cgtctaagaa accattatta tcatgacatt aacctataaa
       61 gataggcgta tcacgaggcc ctttcgtctt cacctcgagt ccctatcagt gatagagatt
      121 gacatcccta tcagtgatag ggatactgag cacatcagca ggacgcactg accgaattca
      181 ttaaagagga gaaaggtacc gcatgaaaat aataataaat aatgatttcc cggtcgctaa
      241 ggtcggagcg gatcaaatta cgactctagt aagtgccaaa gttcatagtt gcatatatcg
      301 gccaagattg agtatcgcgg atggagccgc tcccagagta tgcctttaca gagccccacc
      361 tggatatggg aaaaccgttg ctcttgcgtt cgagtggcta cgccacagaa cagccggacg
      421 tcctgcagtg tggctttctt taagagccag ttcttacagt gaatttgata tctgcgcaga
      481 gattattgag cagcttgaaa ctttcgaaat ggtaaaattc agccgtgtga gagagggtgt
      541 gagcaagcct gcgctcttgc gagaccttgc atctagtctt tggcagagca cctcgaataa
      601 cgagatagaa acgctagttt gtttggataa tattaatcat gacttagact tgccgttgtt
      661 gcacgcactt atggagttta tgttaaatac accaaaaaat atcaggtttg cagttgcagg
      721 caatacaata aaagggttct cgcagcttaa acttgcaggc gctatgcggg agtacaccga
      781 ga
//
