LOCUS       sequence-62676-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Nevozhay D, Adams RM, Murphy KF, Josic K, Balazsi G
  TITLE     Negative autoregulation linearizes the dose-response and suppresses 
            the heterogeneity of gene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 
            10.1073/pnas.0809901106. Epub 2009 Mar 11.
  PUBMED    19279212
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0809901106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-03-31- 31"; volume: "106"; issue: "13"; pages: "5123-8"
COMMENT     Sequence Label: pDN-NG1Tt
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(89..530)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(95..118)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    413..530
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     protein_bind    661..677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 attccgacct cattaagcag ctctaatgcg ctgttaatca ctttactttt atctaatcta
       61 gacattttaa taggatccgg ggttttttct ccttgacgtt aaagtataga ggtatattaa
      121 caattttttg ttgatacttt tattacattt gaataagaag taatacaaac cgaaaatgtt
      181 gaaagtatta gttaaagtgg ttatgcagtt tttgcattta tatatctgtt aatagatcaa
      241 aaatcatcgc ttcgctgatt aattacccca gaaataaggc taaaaaacta atcgcattat
      301 catcctatgg ttgttaattt gattcgttca tttgaaggtt tgtggggcca ggttactgcc
      361 aatttttcct cttcataacc ataaaagcta gtattgtaga atctttattg ttcggagcag
      421 tgcggcgcga ggcacatctg cgtttcagga acgcgaccgg tgaagacgag gacgcacgga
      481 ggagagtctt ccttcggagg gctgtcaccc gctcggcggc ttctaatccg tacttcaata
      541 tagcaatgag cagttaagcg tattactgaa agttccaaag agaaggtttt tttaggctaa
      601 gataatgggg ctctttacat ttccacaaca tataagtaag attagatatg ttgtgtggaa
      661 ttgtgagcgg ataacaattt cacacatgta atatgattat taaacttctt tgcgtccatc
      721 caaaaaaaaa gtaagaattt ttgaaaattc gaattcaacc ctcactaaag ggcggccgca
      781 ctagtgagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat
      841 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta
      901 tgccgccatt attacgacaa gctatcgaat tatt
//
