LOCUS       sequence-62680-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Nevozhay D, Adams RM, Van Itallie E, Bennett MR, Balazsi G
  TITLE     Mapping the environmental fitness landscape of a synthetic gene 
            circuit.
  JOURNAL   PLoS Comput Biol. 2012;8(4):e1002480. doi: 
            10.1371/journal.pcbi.1002480. Epub 2012 Apr 12.
  PUBMED    22511863
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Comput
            Biol."; date: "2012"; volume: "8(4):e1002480. doi"; pages: " 
            10.1371/journal.pcbi.1002480. Epub 2012 Apr 12"
COMMENT     Sequence Label: pDN-T2dGZmxh
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    222..240
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    264..282
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(327..514)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     435..459
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(703..722)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attcttcacc tttagacatt ttaataggat cccccgaatt gatccggtaa tttagtgtgt
       61 gtatttgtgt ttgcgtgtct atagaagtat agtaatttat gctacaaagg acctaatgta
      121 taaggaaaga atatttagag aaaagaagaa aacaagagtt ttatatacat acagagcaca
      181 tgcatgcagt ggtctcgatc gagaaactcg actttgtcga ctctctatca ctgataggga
      241 tcgagaaact cgactttgtc gactctctat cactgatagg gatcgaggaa ttgatctata
      301 ttaccctgtt atccctagcg gatctgccgg tagaggtgtg gtcaataaga gcgacctcat
      361 actatacctg agaaagcaac ctgacctaca ggaaagagtt actcaagaat aagaattttc
      421 gttttaaaac ctaagagtca ctttaaaatt tgtatacact tatttttttt ataacttatt
      481 taataataaa aatcataaat cataagaaat tcgcttattt agaagtggcg ccttaagagc
      541 tccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      601 ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
      661 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
      721 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
      781 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
      841 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtc
//
