LOCUS       sequence-62682-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Nevozhay D, Adams RM, Van Itallie E, Bennett MR, Balazsi G
  TITLE     Mapping the environmental fitness landscape of a synthetic gene 
            circuit.
  JOURNAL   PLoS Comput Biol. 2012;8(4):e1002480. doi: 
            10.1371/journal.pcbi.1002480. Epub 2012 Apr 12.
  PUBMED    22511863
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Comput
            Biol."; date: "2012"; volume: "8(4):e1002480. doi"; pages: " 
            10.1371/journal.pcbi.1002480. Epub 2012 Apr 12"
COMMENT     Sequence Label: pDN-T2dGZmxh
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(141..854)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 gcgaagtcgt cctccacgaa gtcccgggag aacccgagcc ggtcggtcca gaactcgacc
       61 gctccggcga cgtcgcgcgc ggtgagcacc ggaacggcac tggtcaactt ggccatgtta
      121 attaaaccag caccgtcacc tttgtacaat tcatccatac catgggtaat accagcagca
      181 gtaacaaatt ctaacaagac catgtggtct ctcttttcgt ttggatcttt ggataaggca
      241 gattgagtgg ataagtaatg gttgtctggt aacaagactg gaccatcacc aattggagta
      301 ttttgttgat aatggtcagc taattgaaca gaaccatctt caatgttgtg tctaattttg
      361 aagttaactt tgataccatt cttttgtttg tcagccatga tgtaaacatt gtgagagtta
      421 tagttgtatt ccaatttgtg acctaaaatg ttaccatctt ctttaaaatc aatacctttt
      481 aattcgattc tattaactaa ggtatcacct tcaaacttga cttcagctct ggtcttgtag
      541 ttaccgtcat ctttgaaaaa aatagttctt tcttgaacat aaccttctgg catggcagac
      601 ttgaaaaagt catgttgttt catatgatct gggtatctcg caaaacattg aacaccataa
      661 ccgaaagtag tgactaaggt tggccatgga actggcaatt taccagtagt acaaataaat
      721 tttaaggtca atttaccgta agtagcatca ccttcacctt caccggagac agaaaatttg
      781 tgaccattaa catcaccatc taattcaacc aaaattggga caacaccagt gaataattct
      841 tcacctttag acattttaat aggatccccc gaattgatcc g
//
