LOCUS       sequence-62683-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Nevozhay D, Adams RM, Van Itallie E, Bennett MR, Balazsi G
  TITLE     Mapping the environmental fitness landscape of a synthetic gene 
            circuit.
  JOURNAL   PLoS Comput Biol. 2012;8(4):e1002480. doi: 
            10.1371/journal.pcbi.1002480. Epub 2012 Apr 12.
  PUBMED    22511863
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Comput
            Biol."; date: "2012"; volume: "8(4):e1002480. doi"; pages: " 
            10.1371/journal.pcbi.1002480. Epub 2012 Apr 12"
COMMENT     Sequence Label: pDN-T2dMFot
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    250..268
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    292..310
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(355..542)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     463..487
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(726..745)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attaagcagc tctaatgcgc cgttaatcac tttactttta tctaatctag acatggatcc
       61 cccgaattga tccggtaatt tagtgtgtgt atttgtgttt gcgtgtctat agaagtatag
      121 taatttatgc tacaaaggac ctaatgtata aggaaagaat atttagagaa aagaagaaaa
      181 caagagtttt atatacatac agagcacatg catgcagtgg tctcgatcga gaaactcgac
      241 tttgtcgact ctctatcact gatagggatc gagaaactcg actttgtcga ctctctatca
      301 ctgataggga tcgaggaatt gatctatatt accctgttat ccctagcgga tctgccggta
      361 gaggtgtggt caataagagc gacctcatac tatacctgag aaagcaacct gacctacagg
      421 aaagagttac tcaagaataa gaattttcgt tttaaaacct aagagtcact ttaaaatttg
      481 tatacactta ttttttttat aacttattta ataataaaaa tcataaatca taagaaattc
      541 gcttatttag aagtggcgcc ttaagcagct ggcgtaatag cgaagaggcc cgcaccgatc
      601 gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt
      661 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
      721 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
      781 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
      841 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
      901 tcgccctttg acgttggagt ccacgttctt ta
//
