LOCUS       sequence-62693-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Nevozhay D, Zal T, Balazsi G
  TITLE     Transferring a synthetic gene circuit from yeast to mammalian cells.
  JOURNAL   Nat Commun. 2013 Feb 5;4:1451. doi: 10.1038/ncomms2471.
  PUBMED    23385595
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013-02-5"; volume: "4:1451. doi"; pages: " 
            10.1038/ncomms2471"
COMMENT     Sequence Label: pDN-D2irC6kwh
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    221..239
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    247..265
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(261..464)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(290..310)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        465..844
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gcgaaaaaga aagaacaatc aagggtcccc aaactcaccc tgaagttctc aggatcgatc
       61 cggagctttt tgcaaaagcc taggcctcca aaaaagcctc ttcactactt ctggaatagc
      121 tcagaggccc tagaggatcc ccgggtacca agcttaagtt taaacgctag agtccggagg
      181 ctggatcggt cccggtgtct tctatggagg tcaaaacagc tctctatcac tgatagggag
      241 atctgatctc tatcactgat agggatgctt atatagacct cccaccgtac acgcctaccg
      301 cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg
      361 tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca
      421 aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg
      481 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa
      541 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac
      601 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg
      661 aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg
      721 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata
      781 tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa
      841 tgtcaacgcg tatatctggc ccgtacatcg cgaagcagcg caaaacgcct aaccctaagc
      901 agattcttca tgcaattgtc ggtc
//
