LOCUS       sequence-62694-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Nevozhay D, Zal T, Balazsi G
  TITLE     Transferring a synthetic gene circuit from yeast to mammalian cells.
  JOURNAL   Nat Commun. 2013 Feb 5;4:1451. doi: 10.1038/ncomms2471.
  PUBMED    23385595
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013-02-5"; volume: "4:1451. doi"; pages: " 
            10.1038/ncomms2471"
COMMENT     Sequence Label: pDN-D2irC6kwh
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      106..115
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             112..822
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(257..275)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     525..544
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(890..910)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg gcccctctgc
       61 taaccatgtt catgccttct tctttttcct acaggtcctg caggcgccac catggtgagc
      121 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
      181 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
      241 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
      301 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
      361 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
      421 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
      481 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
      541 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
      601 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
      661 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
      721 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
      781 ggccgccact ccaccggcgg catggacgag ctgtacaagt agaccggtgc ggccgcaatc
      841 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
      901 ttacgctatg tggatacgct gctttaatgc ctttg
//
