LOCUS       sequence-62699-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Kaykas A, Moon RT
  TITLE     A plasmid-based system for expressing small interfering RNA 
            libraries in mammalian cells.
  JOURNAL   BMC Cell Biol. 2004 Apr 30;5:16. doi: 10.1186/1471-2121-5-16.
  PUBMED    15119963
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Cell 
            Biol."; date: "2004-04-30"; volume: "5:16. doi"; pages: " 
            10.1186/1471-2121-5-16"
COMMENT     Sequence Label: V43 pHIPPY GFP22
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(112..131)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     680..699
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatcttgtgg ctgcgtctca aaaagagctg tttctgagga gcctttttga gacggtctca
       61 ggatccactt ataagattcc caaatccaaa gacatttcac gtttatggtg atttcccaga
      121 acacatagcg acatgcaaat gcggccgccc actgagcgtc agaccccgtg gaaaagatca
      181 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      241 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      301 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      361 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      421 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      481 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      541 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      601 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      661 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      721 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctat
//
