LOCUS       sequence-62752-e        6807 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Frank S, Gaume B, Bergmann-Leitner ES, Leitner WW, Robert EG, Catez 
            F, Smith CL, Youle RJ
  TITLE     The role of dynamin-related protein 1, a mediator of mitochondrial 
            fission, in apoptosis.
  JOURNAL   Dev Cell. 2001 Oct;1(4):515-25.
  PUBMED    11703942
REFERENCE   2  (bases 1 to 6807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell.";
            date: "2001-10"; volume: "1(4)"; pages: "515-25"
COMMENT     Sequence Label: pEYFP-C1-Drp1
FEATURES             Location/Qualifiers
     source          1..6807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3595..3716
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3632..3651)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3686..3705
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3723..4178)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3860..3881)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4072..4091
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4205..4309
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4311..4668
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4311..4502
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4334..4354)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4519..4654
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4703..5497
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4703..5497
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4757..4776)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5367..5386
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5685..5704)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5729..5776
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6105..6693
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6594..6613
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attcgatgga ggcgctaatt
     1381 cctgtcataa acaagctcca ggacgtcttc aacacggtgg gcgccgacat catccagctg
     1441 cctcaaatcg tcgtagtggg aacgcagagc agcggaaaga gctcagtgct agaaagcctg
     1501 gtggggaggg acctgcttcc cagaggtact ggaattgtca cccggagacc tctcattctg
     1561 caactggtcc atgtttcaca agaagataaa cggaaaacaa caggagaaga aaatggggtg
     1621 gaagcagaag aatggggtaa atttcttcac accaaaaata agctttacac ggattttgat
     1681 gaaattcgac aagaaattga aaatgaaaca gaaagaattt caggaaataa taagggagta
     1741 agccctgaac caattcatct taagattttt tcacccaacg ttgtcaattt gacacttgtg
     1801 gatttgccag gaatgaccaa ggtgcctgta ggtgatcaac ctaaggatat tgagcttcaa
     1861 atcagagagc tcattcttcg gttcatcagt aatcctaatt ccattatcct cgctgtcact
     1921 gctgctaata cagatatggc aacatcagag gcacttaaaa tttcaagaga ggtagatcca
     1981 gatggtcgca gaaccctagc tgtaatcact aaacttgatc tcatggatgc gggtactgat
     2041 gccatggatg tattgatggg aagggttatt ccagtcaaac ttggaataat tggagtagtt
     2101 aacaggagcc agctagatat taacaacaag aagagtgtaa ctgattcaat ccgtgatgag
     2161 tatgcttttc ttcaaaagaa atatccatct ctggccaata gaaatggaac aaagtatctt
     2221 gctaggactc taaacaggtt actgatgcat cacatcagag attgtttacc agagttgaaa
     2281 acaagaataa atgttctagc tgctcagtat cagtctcttc taaatagcta cggtgaaccc
     2341 gtggatgata aaagtgctac tttactccaa cttattacca aatttgccac agaatattgt
     2401 aacactattg aaggaactgc aaaatatatt gaaacttcgg agctatgcgg tggtgctaga
     2461 atttgttata ttttccatga gacttttggg cgaaccttag aatctgttga tccacttggt
     2521 ggccttaaca ctattgacat tttgactgcc attagaaatg ctactggtcc tcgtcctgct
     2581 ttatttgtgc ctgaggtttc atttgagtta ctggtgaagc ggcaaatcaa acgtctagaa
     2641 gagcccagcc tccgctgtgt ggaactggtt catgaggaaa tgcaaaggat cattcagcac
     2701 tgtagcaatt acagtacaca ggaattgtta cgatttccta aacttcatga tgccatagtt
     2761 gaagtggtga cttgtcttct tcgtaaaagg ttgcctgtta caaatgaaat ggtccataac
     2821 ttagtggcaa ttgaactggc ttatatcaac acaaaacatc cagactttgc tgatgcttgt
     2881 gggctaatga acaataatat agaggaacaa aggagaaaca ggctagccag agaattacct
     2941 tcagctgtat cacgagacaa ggttgcatct ggaggtggtg gggttggaga tggtgttcaa
     3001 gaaccaacca caggcaactg gagaggaatg ctgaaaactt caaaagctga agagttatta
     3061 gcagaagaaa aatcaaaacc cattccaatt atgccagcca gtccacaaaa aggtcatgcc
     3121 gtgaacctgc tagatgtgcc agttcctgtt gcacgaaaac tatctgctcg ggaacagcga
     3181 gattgtgagg ttattgaacg actcattaaa tcatattttc tcattgtcag aaagaatatt
     3241 caagacagtg tgccaaaggc agtaatgcat tttttggtta atcatgtgaa agacactctt
     3301 cagagtgagc tagtaggcca gctgtataaa tcatccttat tggatgatct tctgacagaa
     3361 tctgaggaca tggcacagcg caggaaagaa gcagctgata tgctaaaggc attacaagga
     3421 gccagtcaaa ttattgctga aatccgggag actcatcttt ggtgaggatc caccggatct
     3481 agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     3541 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     3601 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     3661 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc
     3721 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     3781 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     3841 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     3901 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     3961 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     4021 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     4081 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     4141 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
     4201 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     4261 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
     4321 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     4381 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     4441 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     4501 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4561 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     4621 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
     4681 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     4741 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     4801 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     4861 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
     4921 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     4981 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     5041 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     5101 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     5161 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     5221 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     5281 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     5341 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     5401 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     5461 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     5521 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     5581 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     5641 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
     5701 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
     5761 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     5821 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     5881 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     5941 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6001 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6061 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6121 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6181 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6241 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6301 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6361 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6421 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6481 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6541 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6601 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6661 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6721 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     6781 gtggataacc gtattaccgc catgcat
//
