LOCUS       sequence-62757-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Scholz J, Besir H, Strasser C, Suppmann S
  TITLE     A new method to customize protein expression vectors for fast, 
            efficient and background free parallel cloning.
  JOURNAL   BMC Biotechnol. 2013 Feb 14;13(1):12.
  PUBMED    23410102
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-02-14"; volume: "13(1)"; pages: "12"
COMMENT     Sequence Label: pCoofy16
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             111..133
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             146..175
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      121 tttaagaagg agatatacca tgaaacatca ccatcaccat caccatcacc atcacaacac
      181 tagtagcaat tccatgaaag aaattttggc tgtagttgaa gccgtatcca atgaaaaggc
      241 gctacctcgc gagaagattt tcgaagcatt ggaaagcgcg ctggcgacag caacaaagaa
      301 aaaatatgaa caagagatcg acgtccgcgt acagatcgat cgcaaaagcg gtgattttga
      361 cactttccgt cgctggttag ttgttgatga agtcacccag ccgaccaagg aaatcaccct
      421 tgaagccgca cgttatgaag atgaaagcct gaacctgggc gattacgttg aagatcagat
      481 tgagtctgtt acctttgacc gtatcactac ccagacggca aaacaggtta tcgtgcagaa
      541 agtgcgtgaa gccgaacgtg cgatggtggt tgatcagttc cgtgaacacg aaggtgaaat
      601 catcaccggc gtggtgaaaa aagtaaaccg cgacaacatc tctctggatc tgggcaacaa
      661 cgctgaagcc gtgatcctgc gcgaagatat gctgccgcgt gaaaacttcc gccctggcga
      721 ccgcgttcgt ggcgtgctct attccgttcg cccggaagcg cgtggcgcgc aactgttcgt
      781 cactcgttcc aagccggaaa tgctgatcga actgttccgt attgaagtgc cagaaatcgg
      841 cgaagaagtg attgaaatta aagcagcggc tcgcgatccg ggttctcgtg cgaaaatcgc
      901 ggtgaaaacc aacgataaac gtatcgatcc ggtaggtgct tgcgtaggta tgcgtggcgc
      961 gcgtgt
//
