LOCUS       sequence-62758-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Osborn AJ, Elledge SJ
  TITLE     Mrc1 is a replication fork component whose phosphorylation in 
            response to DNA replication stress activates Rad53.
  JOURNAL   Genes Dev. 2003 Jul 15;17(14):1755-67.
  PUBMED    12865299
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2003-07-15"; volume: "17(14)"; pages: "1755-67"
COMMENT     Sequence Label: pAO139 (Apr LEU2 mrcl-AQ)
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(20..38)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    80..583
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        620..724
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
       61 ataatggttt cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa
      121 tgatggaata atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat
      181 tttagaaagt aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa
      241 ggtttaaaaa atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc
      301 agattaaata gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg
      361 tgtgtggtct tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa
      421 gagcaagata aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa
      481 aacaaaaact attttttctt taatttcttt ttttactttc tatttttaat ttatatattt
      541 atattaaaaa atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg
      601 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      661 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      721 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      781 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      841 tgcacgagtg ggttacatcg aactggatct ca
//
