LOCUS       sequence-62763-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Scholz J, Besir H, Strasser C, Suppmann S
  TITLE     A new method to customize protein expression vectors for fast, 
            efficient and background free parallel cloning.
  JOURNAL   BMC Biotechnol. 2013 Feb 14;13(1):12.
  PUBMED    23410102
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-02-14"; volume: "13(1)"; pages: "12"
COMMENT     Sequence Label: pCoofy17
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             111..133
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             152..181
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             191..208
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(497..518)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(758..777)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      121 tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcatc atcatcatca
      181 cagcagcggc ctggtgccgc gcggcagcca tatgaatgac cacatcaacc tgaaggtggc
      241 cgggcaggac ggctccgtgg tgcagttcaa gatcaagagg cacacgccgc tgagcaagct
      301 gatgaaggcc tactgcgaga ggcagggctt gtcaatgagg cagatcagat tcaggttcga
      361 cgggcagcca atcaatgaaa ctgacactcc agcacagctg gagatggagg acgaggacac
      421 catcgacgtg ttccagcagc agaccggtgg atccgtgagc aagggcgagg agctgttcac
      481 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      541 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      601 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
      661 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      721 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      781 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      841 cttcaaggag gacggcaaca tcctggggca caagctgga
//
