LOCUS       sequence-62767-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Reed B, Blazeck J, Alper H
  TITLE     Evolution of an alkane-inducible biosensor for increased 
            responsiveness to short-chain alkanes.
  JOURNAL   J Biotechnol. 2012 Apr 15;158(3):75-9. doi: 
            10.1016/j.jbiotec.2012.01.028. Epub 2012 Feb 2.
  PUBMED    22326628
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jbiotec.2012.01.028"; journalName: "J Biotechnol"; date: 
            "2012-04-15- 15"; volume: "158"; issue: "3"; pages: "75-9"
COMMENT     Sequence Label: pACYC-PLlambda-alkSm4-3
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(98..116,117..122,123..139,140..145,146..171)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    98..116
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             181..192
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 atttctagac aattccgacg tctaagaaac cattattatc atgacattaa cctataaaga
       61 taggcgtatc acgaggccct ttcgtcttca cctcgagtcc ctatcagtga tagagattga
      121 catccctatc agtgataggg atactgagca catcagcagg acgcactgac cgaattcatt
      181 aaagaggaga aaggtaccgc atgaaaataa taataaataa tgatttcccg gtcgctaagg
      241 tcggagcgga tcaaattacg actctagtaa gtgccaaagt tcatagttgc atatatcggc
      301 caagattgag tatcgcggat ggagccgctc ccagagtatg cctttacaga gccccacctg
      361 gatatgggaa aaccgttgct cttgcgttcg agtggctacg ccacagaaca gccggacgtc
      421 ctgcagtgtg gctttcttta agagccagtt cttacagtga atttgatatc tgcgcagaga
      481 ttattgagca gcttgaaact ttcgaaatgg taaaattcag ccgtgtgaga gagggtgtga
      541 gcaagcctgc gctcttgcga gaccttgcat ctagtctttg gcagagcacc tcgaataacg
      601 agatagaaac gctagtttgt ttggataata ttaatcatga cttagacttg ccgttgttgc
      661 acgcacttat ggagtttatg ttaaatacac caaaaaatat caggtttgca gttgcaggca
      721 atacaataaa agggttctcg cagcttaaac ttgcaggcgc tatgcgggag tacaccgaga
//
