LOCUS       sequence-62776-e         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Scholz J, Besir H, Strasser C, Suppmann S
  TITLE     A new method to customize protein expression vectors for fast, 
            efficient and background free parallel cloning.
  JOURNAL   BMC Biotechnol. 2013 Feb 14;13(1):12.
  PUBMED    23410102
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-02-14"; volume: "13(1)"; pages: "12"
COMMENT     Sequence Label: pCoofy26
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             111..133
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      121 tttaagaagg agatatacca tgaaaaacac tagtagcaat tccatgaaag aaattttggc
      181 tgtagttgaa gccgtatcca atgaaaaggc gctacctcgc gagaagattt tcgaagcatt
      241 ggaaagcgcg ctggcgacag caacaaagaa aaaatatgaa caagagatcg acgtccgcgt
      301 acagatcgat cgcaaaagcg gtgattttga cactttccgt cgctggttag ttgttgatga
      361 agtcacccag ccgaccaagg aaatcaccct tgaagccgca cgttatgaag atgaaagcct
      421 gaacctgggc gattacgttg aagatcagat tgagtctgtt acctttgacc gtatcactac
      481 ccagacggca aaacaggtta tcgtgcagaa agtgcgtgaa gccgaacgtg cgatggtggt
      541 tgatcagttc cgtgaacacg aaggtgaaat catcaccggc gtggtgaaaa aagtaaaccg
      601 cgacaacatc tctctggatc tgggcaacaa cgctgaagcc gtgatcctgc gcgaagatat
      661 gctgccgcgt gaaaacttct gccctggcga ccgcgttcgt ggcgtgctct attccgttcg
      721 cccggaagcg cgtggcgcgc aactgttcgt cactcgttcc aagccggaaa tgctgatcga
      781 actgttccgt attgaagtgc cagaaatcgg cgaagaagtg attgaaatta aagcagcggc
      841 tcgcgatccg ggttctcgtg cgaaaatcgc ggtgaaaacc aacgataaac gtatcgatcc
      901 ggtaggtgct tgcgtaggta tgcgtggcgc gcgtgttcag gcggtgtcta ctgaactggg
      961 tg
//
