LOCUS       sequence-62779-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 
            promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Hwang WY, Fu Y, Reyon D, Maeder ML, Tsai SQ, Sander JD, Peterson RT,
            Yeh JR, Joung JK
  TITLE     Efficient genome editing in zebrafish using a CRISPR-Cas system.
  JOURNAL   Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501.
  PUBMED    23360964
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501."
COMMENT     Sequence Label: MLM3613
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(46..66)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggtgatggtg atgatgaccg gtcatcctgc agctccaccg ctcgagactt tcctcttctt
       61 cttgggagaa ccgtcacctc ctagctgact caaatcaatg cgtgtttcat aaagaccagt
      121 gatggattga tggataagag tggcatctaa aacttctttt gtagacgtat atcgtttacg
      181 atcaattgtt gtatcaaaat atttaaaagc agcgggagct ccaagattcg tcaacgtaaa
      241 taaatgaata atattttctg cttgttcacg tattggtttg tctctatgtt tgttatatgc
      301 actaagaact ttatctaaat tggcatctgc taaaataaca cgcttagaaa attcactgat
      361 ttgctcaata atctcatcta aataatgctt atgctgctcc acaaacaatt gtttttgttc
      421 gttatcttct ggactaccct tcaacttttc ataatgacta gctaaatata aaaaattcac
      481 atatttgctt ggcagagcca gctcatttcc tttttgtaat tctccggcac tagccagcat
      541 ccgtttacga ccgttttcta actcaaaaag actatattta ggtagtttaa tgattaagtc
      601 ttttttaact tccttatatc ctttagcttc taaaaagtca atcggatttt tttcaaagga
      661 acttctttcc ataattgtga tccctagtaa ctctttaacg gattttaact tcttcgattt
      721 ccctttttcc accttagcaa ccactaggac tgaataagct accgttggac tatcaaaacc
      781 accatatttt tttggatccc agtctttttt acgagcaata agcttgtccg aatttctttt
      841 tggtaaaatt gactccttgg
//
