LOCUS       sequence-62805-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Blazeck J, Liu L, Redden H, Alper H
  TITLE     Tuning gene expression in Yarrowia lipolytica by a hybrid promoter 
            approach.
  JOURNAL   Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 
            10.1128/AEM.05763-11. Epub 2011 Sep 16.
  PUBMED    21926196
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.05763-11"; journalName: "Appl Environ Microbiol"; date:
            "2011-11"; volume: "77"; issue: "22"; pages: "7905-14"
COMMENT     Sequence Label: pUC-Leum
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(147..164)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(147..163)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(156..178)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(372..391)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     491..513
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(551..569)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        637..741
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atgtgtgtgg ttgtatgtgt gatgtggtgt gtggagtgaa aatctgtggc tggcaaacgc
       61 tcttgtatat atacgcactt ttgcccgtga tcagtgcatg cctgcaggtc gactctagag
      121 gatccccggg taccgagctc gaattcactg gccgtcgttt tacaacgtcg tgactgggaa
      181 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
      241 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
      301 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
      361 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
      421 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
      481 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
      541 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
      601 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      661 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
      721 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
      781 tttgcggcat
//
