LOCUS       sequence-62829-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Blazeck J, Liu L, Redden H, Alper H
  TITLE     Tuning gene expression in Yarrowia lipolytica by a hybrid promoter 
            approach.
  JOURNAL   Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 
            10.1128/AEM.05763-11. Epub 2011 Sep 16.
  PUBMED    21926196
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.05763-11"; journalName: "Appl Environ Microbiol"; date:
            "2011-11"; volume: "77"; issue: "22"; pages: "7905-14"
COMMENT     Sequence Label: pUC-UAS1B5-Leum
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(677..694)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(677..693)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(686..708)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtttcgggtt ccatttgtgg atgtgtgtgg ttgtatgtgt gatgtggtgt gtggagtgaa
       61 aatctgtggc tggcaaacgc tcttgtatat atacgcactt ttgcccgtga tcagtgcatg
      121 cccggatcga ggtgggcggg aacaacggtc tcgataatgt acgtacacta cacacaaaga
      181 gaagcaagtg ggggcgggac tgcacggcac ttgtgagaca cctcaggagc tcccggatcg
      241 aggtgggcgg gaacaacggt ctcgataatg tacgtacact acacacaaag agaagcaagt
      301 gggggcggga ctgcacggca cttgtgagac acctcaggtc gacccggatc gaggtgggcg
      361 ggaacaacgg tctcgataat gtacgtacac tacacacaaa gagaagcaag tgggggcggg
      421 actgcacggc acttgtgaga cacctcaggg atccccggat cgaggtgggc gggaacaacg
      481 gtctcgataa tgtacgtaca ctacacacaa agagaagcaa gtgggggcgg gactgcacgg
      541 cacttgtgag acacctcagt ctagaccgga tcgaggtggg cgggaacaac ggtctcgata
      601 atgtacgtac actacacaca aagagaagca agtgggggcg ggactgcacg gcacttgtga
      661 gacacctcag gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
      721 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcg
//
