LOCUS       sequence-62834-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Blonska M, Shambharkar PB, Kobayashi M, Zhang D, Sakurai H, Su B, 
            Lin X
  TITLE     TAK1 is recruited to the tumor necrosis factor-alpha (TNF-alpha) 
            receptor 1 complex in a receptor-interacting protein (RIP)-dependent
            manner and cooperates with MEKK3 leading to NF-kappaB activation.
  JOURNAL   J Biol Chem. 2005 Dec 30;280(52):43056-63. Epub 2005 Oct 31.
  PUBMED    16260783
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2005-12-30"; volume: "280(52)"; pages: "43056-63. 
            Epub 2005 Oct 31"
COMMENT     Sequence Label: pRK6-HA-TAK1
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..31
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        241..259
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     241..258
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     272..291
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             340..366
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
       61 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
      121 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
      181 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
      241 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
      301 caggtccaac tgcacctcgg ttctatcgat gccaccatgt acccatacga tgttccagat
      361 tacgctggat ccatgtcgac agcctccgcc gcctcgtcct cctcctcgtc ttctgccagt
      421 gagatgatcg aagcgccgtc gcaggtcctg aacttcgaag agatcgacta caaggagatc
      481 gaggtggaag aggttgtcgg aagaggagct tttggagtag tttgcaaagc taagtggaga
      541 gcaaaagatg tcgctattaa acagatagaa agtgagtctg agaggaaggc tttcattgtg
      601 gagctccggc agttgtcgcg tgtgaaccat cctaacattg tcaagttgta cggagcctgc
      661 ctgaatccag tatgtcttgt gatggaatat gcagaggggg gctcattgta taatgtgctg
      721 catggtgctg aaccattgcc ttactacact gctgctcatg ccatgagctg gtgtttacag
      781 tgttcccaag gagtggctta cctgcacagc atgcagccca aagcgctgat tcacagggac
      841 ctcaagcctc caaacttgct gctggttgca ggagggacag ttctaaaaat ctgcgatttt
      901 ggtacagctt gtgacatcca aacacacatg
//
