LOCUS       sequence-62842-e        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Sathiamoorthy S, Shin JA
  TITLE     Boundaries of the origin of replication: creation of a 
            pET-28a-derived vector with p15A copy control allowing compatible 
            coexistence with pET vectors.
  JOURNAL   PLoS One. 2012;7(10):e47259. doi: 10.1371/journal.pone.0047259. Epub
            2012 Oct 22.
  PUBMED    23110063
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(10):e47259. doi"; pages: " 
            10.1371/journal.pone.0047259. Epub 2012 Oct 22"
COMMENT     Sequence Label: pSAM
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(237..254)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(371..915)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(451..470)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 caaaggatct tcttgagatc ctggcttctg tttctatcag ctgtccctcc tgttcagcta
       61 ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca tcagcgctag cggagtgtat
      121 actggcttac tatgttggca ctgatgaggg tgtcagtgaa gtgcttcatg tggcaggaga
      181 aaaaaggctg caccggtgcg tcagcagaat atgtgataca ggatatattc cgcttcctcg
      241 ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg
      301 ggcggagatt tcctggaaga tgccaggaag atacttaaca gggaagtgag agggccgcgg
      361 caaagccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa
      421 atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc ctggcggctc
      481 cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc
      541 gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg
      601 actgtatgca cgaacccccc gttcagtccg accgctgcgc cttatccggt aactatcgtc
      661 ttgagtccaa cccggaaaga catgcaaaag caccactggc agcagccact ggtaattgat
      721 ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt
      781 ggtgactgcg ctcctccaag ccagttacct cggttcaaag agttggtagc tcagagaacc
      841 ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag agcaagagat tacgcgcaga
      901 ccaaaacgat ctcaagaaga tcatcttatt aatcagataa aatatttcta gatttcagtg
      961 caatttatct cttcaaatgt agcacctgaa gtcagcccca tacgatataa gttgtaattc
     1021 tcatgttagt catgccccgc gaaacgagag aggatgctca cg
//
