LOCUS       sequence-62848-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Blazeck J, Liu L, Redden H, Alper H
  TITLE     Tuning gene expression in Yarrowia lipolytica by a hybrid promoter 
            approach.
  JOURNAL   Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 
            10.1128/AEM.05763-11. Epub 2011 Sep 16.
  PUBMED    21926196
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.05763-11"; journalName: "Appl Environ Microbiol"; date:
            "2011-11"; volume: "77"; issue: "22"; pages: "7905-14"
COMMENT     Sequence Label: pUC-UAS1B4-Leum
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(571..588)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(571..587)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(580..602)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtttcgggtt ccattgtgga tgtgtgtggt tgtatgtgtg atgtggtgtg tggagtgaaa
       61 atctgtggct ggcaaacgct cttgtatata tacgcacttt tgcccgtgat cagtgcatgc
      121 ccggatcgag gtgggcggga acaacggtct cgataatgta cgtacactac acacaaagag
      181 aagcaagtgg gggcgggact gcacggcact tgtgagacac ctcaggtcga cccggatcga
      241 ggtgggcggg aacaacggtc tcgataatgt acgtacacta cacacaaaga gaagcaagtg
      301 ggggcgggac tgcacggcac ttgtgagaca cctcagggat ccccggatcg aggtgggcgg
      361 gaacaacggt ctcgataatg tacgtacact acacacaaag agaagcaagt gggggcggga
      421 ctgcacggca cttgtgagac acctcagtct agaccggatc gaggtgggcg ggaacaacgg
      481 tctcgataat gtacgtacac tacacacaaa gagaagcaag tgggggcggg actgcacggc
      541 acttgtgaga cacctcagga gctcgaattc actggccgtc gttttacaac gtcgtgactg
      601 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
      661 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
      721 cgaatgg
//
