LOCUS       sequence-62849-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  TITLE     Formosa lab yeast 3' tagging constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTF258 (pFA6a-PSc-6xGly-His3MX)
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(84..281)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(575..589)
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
ORIGIN
        1 gcataggcca ctagtggatc tgatatcatc gatgaattcg agctcgttta aactggatgg
       61 cggcgttagt atcgaatcga cagcagtata gcgaccagca ttcacatacg attgacgcat
      121 gatattactt tctgcgcact taacttcgca tctgggcaga tgatgtcgag gcgaaaaaaa
      181 atataaatca cgctaacatt tgattaaaat agaacaacta caatataaaa aaactataca
      241 aatgacaagt tcttgaaaac aagaatcttt ttattgtcag tacttcacat caaaacacct
      301 ttggttgagg gaacgtcatt ggtgccattg ctagaaatag cttctcttat ggcaacagcc
      361 aaagccttga acgcactctc acttctgtgg tgatcgttga aacctctcag acaatcaaca
      421 tgcaaagtaa ttctggccgc ctccgcgaaa ctttccaaaa agtgtggaat catttcagtg
      481 gataaatcac caatcatctc tctcttcaat ccaaggtcga ttacagcaaa tggtctacta
      541 gataaatcga ctacggcacg tgatagcgcc tcatccaatg gtgcgaaccc agtaccgaat
      601 ctttttacac cacggacagc acccattgct tctttgaacg cttgccctaa tgcgataccg
      661 caatcttcgg tagtatggtg atcgtcaatg tgcaggtcac caatacattc aacaataaga
      721 gaccaaccag agtgttttgc caacgcatgg atcatatgat ccaaaaagcc aacacctgtg
      781 tgaatatcga tgacctgtga ctgagtagct tgggaagcta catcgtcatc cttctttgca
      841 ggaagaatcg aatcttttat ttgaatata
//
