LOCUS       sequence-62851-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  TITLE     Matt Scott lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUASP Flag Rab32 WT
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      235..244
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             244..267
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    328..352
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cttaaattca gtgcacgttt gcttgttgag aggaaaggtt gtgtgcggac gaattttttt
       61 ttgaaaaccg gtgatagagc ctgaaccaga aaagataaaa gaaggctata ccagtgggag
      121 tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca ataacgtgac
      181 tgtgcgttag gtcctgttca ttggtacccg cccggggatc agatccgcgg ccgcgccacc
      241 atggattaca aggatgacga cgataagaga tctcgagctc aagcttcgaa ttctgcagtc
      301 gacggtaccg cgggcccggg atcatcaaca agtttgtaca aaaaagcagg cttaatgaca
      361 ctaagatcca aagccatcat gacatccacc agcgataagc gcgagcatct gtacaagatt
      421 ctagtcattg gtgaactggg caccggcaag acgtccttca ttaagcgcta tgtgcaccag
      481 ttcttcagcc agaactacag ggccaccatc ggcgtggact tcgccctgaa agtgctgcag
      541 tgggacgcca atacgatagt gcgcctccag ctgtgggata tcgccggtca ggagaggttt
      601 ggcaacatga cccgtgtcta ctacaaggag gcggtgggcg cgttcatcgt ttttgatgtg
      661 actcgcagtg ggactttcga ttgcgtgagc aagtggaagg aagatctaga ctccaaggtg
      721 cagctgccgg acggcagtcc cattccgtgc atcctgctgg ccaataaatg cgaccaggaa
      781 aagcagggca tcataacgca gcccgagaag atggatgagt atgtgaggga gaacggattt
      841 gccggttggt ttgagacatc cgccaaggag aatatcaaca tc
//
