LOCUS       sequence-62855-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  TITLE     Formosa lab yeast 3' tagging constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pZC04 (pFA6a-PSc-6xGly-PrtA-His3MX)
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(86..283)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(577..591)
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
ORIGIN
        1 ccgcataggc cactagtgga tctgatatca tcgatgaatt cgagctcgtt taaactggat
       61 ggcggcgtta gtatcgaatc gacagcagta tagcgaccag cattcacata cgattgacgc
      121 atgatattac tttctgcgca cttaacttcg catctgggca gatgatgtcg aggcgaaaaa
      181 aaatataaat cacgctaaca tttgattaaa atagaacaac tacaatataa aaaaactata
      241 caaatgacaa gttcttgaaa acaagaatct ttttattgtc agtacttcac atcaaaacac
      301 ctttggttga gggaacgtca ttggtgccat tgctagaaat agcttctctt atggcaacag
      361 ccaaagcctt gaacgcactc tcacttctgt ggtgatcgtt gaaacctctc agacaatcaa
      421 catgcaaagt aattctggcc gcctccgcga aactttccaa aaagtgtgga atcatttcag
      481 tggataaatc accaatcatc tctctcttca atccaaggtc gattacagca aatggtctac
      541 tagataaatc gactacggca cgtgatagcg cctcatccaa tggtgcgaac ccagtaccga
      601 atctttttac accacggaca gcacccattg cttctttgaa cgcttgccct aatgcgatac
      661 cgcaatcttc ggtagtatgg tgatcgtcaa tgtgcaggtc accaatacat tcaacaataa
      721 gagaccaacc agagtgtttt gccaacgcat ggatcatatg atccaaaaag ccaacacctg
      781 tgtgaatatc gatgacctgt gactgagtag cttgggaagc tacatcgtca tccttctttg
      841 caggaagaat cgaatctttt atttgaatat aaccaccatt cagcga
//
