LOCUS       sequence-62857-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  TITLE     Formosa lab yeast 3' tagging constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pZC09 (pFA6a-TEV-6xGly-PrtA-His3MX)
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(87..284)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(578..592)
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
ORIGIN
        1 gccgcatagg ccactagtgg atctgatatc atcgatgaat tcgagctcgt ttaaactgga
       61 tggcggcgtt agtatcgaat cgacagcagt atagcgacca gcattcacat acgattgacg
      121 catgatatta ctttctgcgc acttaacttc gcatctgggc agatgatgtc gaggcgaaaa
      181 aaaatataaa tcacgctaac atttgattaa aatagaacaa ctacaatata aaaaaactat
      241 acaaatgaca agttcttgaa aacaagaatc tttttattgt cagtacttca catcaaaaca
      301 cctttggttg agggaacgtc attggtgcca ttgctagaaa tagcttctct tatggcaaca
      361 gccaaagcct tgaacgcact ctcacttctg tggtgatcgt tgaaacctct cagacaatca
      421 acatgcaaag taattctggc cgcctccgcg aaactttcca aaaagtgtgg aatcatttca
      481 gtggataaat caccaatcat ctctctcttc aatccaaggt cgattacagc aaatggtcta
      541 ctagataaat cgactacggc acgtgatagc gcctcatcca atggtgcgaa cccagtaccg
      601 aatcttttta caccacggac agcacccatt gcttctttga acgcttgccc taatgcgata
      661 ccgcaatctt cggtagtatg gtgatcgtca atgtgcaggt caccaataca ttcaacaata
      721 agagaccaac cagagtgttt tgccaacgca tggatcatat gatccaaaaa gccaacacct
      781 gtgtgaatat cgatgacctg tgactgagta gcttgggaag ctacatcgtc atccttcttt
      841 g
//
