LOCUS       sequence-62865-e       12683 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12683)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 12683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pThy1-Brainbow3.0
FEATURES             Location/Qualifiers
     source          1..12683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6281..6305
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6354..6387)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(6396..6429)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      6438..6447
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6444..7151
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    7333..7454
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(7467..7500)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      7509..7518
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7515..7517,7518..7520,7521..8231)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7515..7517,7518..7520,7521..8231)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7560..7581)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7821..7840)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8168..8189
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8413..8534
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(8551..8584)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      8593..8602
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8599..9294
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    9476..9597
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    9610..9643
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(9682..9706)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(12514..12533)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12633..12655
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcaggatc gcggccgcga tccccgggcg agctcgaatt agttacgcta
     2101 gggataacag ggtaatatag aaaattcaga gaccgggaac caaactagcc tttaaaaaac
     2161 ataagtacag gagccagcaa gatggctcag tgggtaaagg tgcctaccag caagcctgac
     2221 agcctgagtt cagtccccac gaactacgtg gtaggagagg accaaccaac tctggaaatc
     2281 tgttctgcaa acacatgctc acacacacac acacaaatag tataaacaat tttaaatttc
     2341 atttaaaaat aatttgtaaa caaaatcatt agcacaggtt ttagaaagag cctcttggtg
     2401 acatcaagtt gatgctgtag atggggtatc attcctgagg acccaaaacc gggtctcagc
     2461 ctttccccat tctgagagtt ctctcttttc tcagccacta gctgaagagt agagtggctc
     2521 agcactgggc tcttgagttc ccaagtccta caactggtca gcctgactac taaccagcca
     2581 tgaagaaaca aggagtggat gggctgagtc tgctgggatg ggagtggagt tagtaagtgg
     2641 ccatggatgt aatgacccca gcaatgctgg ctagaaggca tgcctccttt ccttgtctgg
     2701 agacggaacg ggatcatctt gtactcacag aagggagaac attctagctg gttgggccaa
     2761 aatgtgcaag ttcacctgga ggtggtggtg catgctttta actccagtac tcaggaggca
     2821 gggccaggtg gatctctgtg agttcaagac cagcctgcac tatggagaga gttttgggac
     2881 agccagagtt acacagaaaa atcctggtgg aaaatctgaa agaaagagag aaagaaagaa
     2941 agaaagaaag aaagaaagaa agaaagaaag aaagaaagaa agaaagaaag aaaggaagaa
     3001 agaaagaaag agtggcaggc aggcaggcag gaggaaggaa ggaaggaagg aaggaaggaa
     3061 ggaaggaagg aaggaaggaa ggaaggaagg aaggaaaata ggtgcgactt caagatccgg
     3121 agttacaagc agaatgcact gtttccctaa cagggccaag tgttttgagt aactgaaggt
     3181 gggcatgatg cctgggaagc agaaacaagc caggcagatg caccccttgc cttgcttccg
     3241 aagggctgca gtagcatgga aaacatggaa aacaaccaat ccattccctt tgctgatata
     3301 acaggctcca aagccaaaac ctgtcactgg aggctcaaga gcagatctcc agccaagagg
     3361 caaaggaatg ggggaagctg gagggcctcc ctctggttat ccaggcttct gaaggttcaa
     3421 gcaaagaaag ggttacaacc ttaaaaggag agcgtcccgg ggtatgggta gaagactgct
     3481 ccaccccgac ccccagggtc cctaaccgtc ttttccctgg gcgagtcagc ccaatcacag
     3541 gactgagagt gcctctttag tagcagcaag ccacttcgga cacccaaatg gaacacctcc
     3601 agtcagccct cgccgaccac cccaccccct ccatcctttt ccctcagcct ccgattggct
     3661 gaatctagag tccctccctg ctcccccctc tctccccacc cctggtaaaa ctgcgggctt
     3721 caggctgggt gcagcaactg gaggcgttgg cgaccaggag gaggctgcag ctaggggagt
     3781 ccaggtgaga gcaggccgac gggagggacc cgcacatgca aggaccgccg cagggcgagg
     3841 atgcaagcct tccccagcta cagttttggg aaaggatacc agggcgctcc tatatggggg
     3901 cgcgggaacc tggggaaaga aggtgctccc aggtcgaggt gggagaggaa ggcagtgcgg
     3961 ggtcacgggc tttctccctg ctaacggacg ctttcgaaga gtgggtgccg gagagaacca
     4021 tgaggaagga catcaaggac agcctttggt ccccaagctc aaatcgcttt agtggtgcga
     4081 atagagggag gaggtgggtg gcaaactgga gggagtcccc agcggtgacc tcgtggctgg
     4141 ctggtgcggg gcaccaggta agaaacgcaa tgttgcggga ggggactggg tggcaggcgc
     4201 gggggagggg aaagctagaa aggatgcgag ggagcggagg ggggagggag cgggggaatc
     4261 tcaactggta gaggaaagtt aaaatgagga aatagcatca gggtggggtt agccaagccg
     4321 ggcctcaggg aaagggcgca aagtttgtct gggtgtgggc ttaggtgggc tgggtatgag
     4381 attcggggcg ccgaaaacac tgctgcgcct ctgccaaatc acgctacccc tgtatctagt
     4441 tctgctaggc ttctccagcc ccagccccaa ttcttttctc agtgtcccct tccctcccct
     4501 gaatctcaag cccacactcc ctcctccata acccactgtt atcaaatcta agtcatttgc
     4561 cacccaacaa ccatcaggag gcggaagcag acgggaggag tttgagatca acttgggcta
     4621 catcacgagt tccaagctca ccaaggcttc ttaaggagac cttgtctcta aaattaatta
     4681 attaattaat taatagtccc ctttctctgc cacagaacct tgggatctgg ctcctggtcg
     4741 cagctccccc caccccaggc tgacattcac tgccatagcc catccggaaa tcctagtcta
     4801 ttccccatgg atcttgaact gcagagagaa tggcagagtg gcccgccctg tgcaaaggat
     4861 gttcctagcc taggtggagc tcgcgaactc ggagactgtg cctctcttgg gcaaggacag
     4921 gctagacagc ctgccggtgt tgagctaggg cactgtgggg aaggcagaga acctgtgcag
     4981 ggcacgcaat gaacacagga ccagaaaact gcagccctag gaacactcaa gagctggcca
     5041 tttgcaagca tctctggcct ccgtgcttct cactcatgtc ccatgtctta tacaggcctc
     5101 tgtggcacct cgcttgcctg atctcatccc tagccgttaa gctttctgca tgacttatca
     5161 cttggggcat aatgctggat acctaccatt ttcttagacc ccatcaaaat cctatttgag
     5221 tgtacggttc ggagaactcc ttatttatcc ggtaaatgtc ttttactctg ctctcaggga
     5281 gctgaggcag gacattcctg agatacattg ggagaggaat acagtttcaa taaaataata
     5341 ggttgggtgg aggtacatgc ctataatgcc accactcagg aaatggtggc agcttcgtga
     5401 gtttgaggcc aacccaagaa acatagtgaa accctgtcag taaataagta agcaagtatt
     5461 tgagtatcta ctatatgcta gggctgacct ggacattagg ggtcatcttc tgaacaaact
     5521 agtgcttgag ggaggtattt ggggtttttg tttgtttaat ggatctgaat gagttccaga
     5581 gactggctac acagcgatat gactgagctt aacaccccta aagcatacag tcagaccaat
     5641 tagacaataa aaggtatgta tagcttacca aataaaaaaa ttgtattttc aagagagtgt
     5701 ctgtctgtgt agccctggct gttcttgaac tcactctgta gaccaggctg gcctggaaat
     5761 ccatctgcct ctgcctctct gcctctctgc ctctctgcct ctctctctgc ctctctctgc
     5821 ctctctctgc ccctctctgc ccctctctgc ccctctctgc cgccctctgc cttttgccct
     5881 ctgccctctg ttctctggcc tctgccctct ccctgccttc tccctctgcc tctgcctctt
     5941 gagtgctgga atcaaaggtg tgagctctat aggtcttaag ttccagaaga aacgtaatga
     6001 agtcacccag caggaggtgc tcagggacag caagacacac acacccagga cactaggctc
     6061 ccacttcctt ggctttctct gagtggcaaa ggaccttagg cagtgtcact ccctaagaga
     6121 aggggataaa gagaggggct gaggtattca tcatgtgctc cgtggatctc aagccctcaa
     6181 ggtaaatggg gacccacctg tcctaccagc tggctgacct gtagctttcc ccaccacaga
     6241 atccaagtcg gaactcttgg cacctagagg atctcgaatc acaagtttgt acaaaaaagc
     6301 aggctttaaa ggaacgcacg taactataac ggtcctaagg tagcgaacct aggataactt
     6361 cgtatagcat acattatacg aagttatcgg cgcgcataac ttcgtatagg atactttata
     6421 cgaagttatc aagcgctgcc accatggtga gcaagggcga ggagaataac atggccatca
     6481 tcaaggagtt catgcgcttc aaggtgcgca tggagggctc cgtgaacggc cacgagttcg
     6541 agatcgaggg cgagggcgag ggccgcccct acgagggctt tcagaccgct aagctgaagg
     6601 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcat ttcacctacg
     6661 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttcaag ctgtccttcc
     6721 ccgagggctt caagtgggag cgcgtgatga actacgagga cggcggcgtg gtgaccgtga
     6781 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     6841 acttcccctc cgacggcccc gtgatgcaga agaagaccat gggctgggag gcctcctccg
     6901 agcggatgta ccccgaggac ggtgccctga agggcaagat caagatgagg ctgaagctga
     6961 aggacggcgg ccactacacc tccgaggtca agaccaccta caaggccaag aagcccgtgc
     7021 agctgcccgg cgcctacatc gtcgacatca agttggacat cacctcccac aacgaggact
     7081 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     7141 agctgtacaa gaagctgaac cctcctgatg agagtggccc cggctgcatg agctgctgtg
     7201 tgctctcctg agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt
     7261 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     7321 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     7381 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     7441 atcaatgtat cttaaggcgt gctagcataa cttcgtatag catacattat acgaagttat
     7501 ctaacgttgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     7561 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     7621 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     7681 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     7741 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     7801 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     7861 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     7921 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     7981 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     8041 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     8101 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     8161 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     8221 agctgtacaa gaagctgaac cctcctgatg agagtggccc cggctgcatg agctgctgtg
     8281 tgctctcctg agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt
     8341 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     8401 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8461 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8521 atcaatgtat cttaaggcgt gtttaaacat ataacttcgt ataggatact ttatacgaag
     8581 ttatcgcgta cggccaccat ggtgagcgag ctgattaagg agaacatgca catgaagctg
     8641 tacatggagg gcaccgtgaa caaccaccac ttcaagtgca catccgaggg cgaaggcaag
     8701 ccctacgagg gcacccagac catgagaatc aaggcggtcg agggcggccc tctccccttc
     8761 gccttcgaca tcctggctac cagcttcatg tacggcagca aaaccttcat caaccacacc
     8821 cagggcatcc ccgacttctt taagcagtcc ttccccgagg gcttcacatg ggagagagtc
     8881 accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc
     8941 tgcctcatct acaacgtcaa gatcagaggg gtgaacttcc catccaacgg ccctgtgatg
     9001 cagaagaaaa cactcggctg ggaggcctcc accgagaccc tgtaccccgc tgacggcggc
     9061 ctggaaggca gagccgacat ggccctgaag ctcgtgggcg ggggccacct gatctgcaac
     9121 ttgaagacca catacagatc caagaaaccc gctaagaacc tcaagatgcc cggcgtctac
     9181 tatgtggaca gaagactgga aagaatcaag gaggccgaca aagagaccta cgtcgagcag
     9241 cacgaggtgg ctgtggccag atactgcgac ctccctagca aactggggca cagaaagctg
     9301 aaccctcctg atgagagtgg ccccggctgc atgagctgct gtgtgctctc ctgagcggcc
     9361 gcgactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     9421 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     9481 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     9541 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg
     9601 cgtactagtg aagttcctat tctctagaaa gtataggaac ttccgtaact ataacggtcc
     9661 taaggtagcg aatgcatcta gacccagctt tcttgtacaa agtggtgatt cgaggtcctt
     9721 cctctgcaga ggtcttgctt ctcccggtca gctgactccc tccccaagtc cttcaaatat
     9781 ctcagaacat ggggagaaac ggggaccttg tccctcctaa ggaaccccag tgctgcatgc
     9841 catcatcccc cccaccctcg cccccacccc cgccacttct ccctccatgc ataccactag
     9901 ctgtcatttt gtactctgta tttattctag ggctgcttct gattatttag tttgttcttt
     9961 ccctggagac ctgttagaac ataagggcgt atggtgggta ggggaggcag gatatcagtc
    10021 ccctggggcg agttcctccc tgccaaccaa gccagatgcc tgaaagagat atggatgagg
    10081 gaagttggac tgtgcctgta cctggtacag tcatactctg ttgaaagaat catcggggag
    10141 gggggggggc tcaagagggg agagctctct gagcctttgt ggaccatcca atgaggatga
    10201 gggcttagat tctaccaggt cattctcagc caccacacac aagcgctctg ccatcactga
    10261 agaagccccc tagggctctt gggccagggc acactcagta aagatgcagg ttcagtcagg
    10321 gaatgatggg gaaaggggta ggaggtgggg gagggatcac cccctcctct aaaacacgag
    10381 cctgctgtct ccaaaggcct ctgcctgtag tgagggtggc agaagaagac aaggagccag
    10441 aactctgact ccaggatcta agtccgtgca ggaaggggat cctagaacca tctggttgga
    10501 cccagcttac caagggagag cctttattcc ttctttccct tgcccctctg tgccagcccc
    10561 tcttgctgtc cctgatcccc cagacagcga gagtcttgca acctgcctct tccaagacct
    10621 cctaatctca ggggcaggcg gtggagtgag atccggcgtg cacacttttt ggaagatagc
    10681 tttcccaagg atcctctccc ccactggcag ctctgcctgt cccatcacca tgtataatac
    10741 caccactgct acagcatctc accgaggaaa gaaaactgca caataaaacc aagcctctgg
    10801 agtgtgtcct ggtgtctgtc tcttctgtgt cctggcgtct gtctcttctg tgttcttcca
    10861 aggtcagaaa caaaaaccac acacttcaac ctggatggct cggctgagca cttctgtgtg
    10921 cagaaggtcc aaccagactc tggggtaccc cggccctccc tattcccttg cctcctgtct
    10981 cccgcttttt atagctccct atgctgggct tctctggaga gtgaaatctt tgcccaaatc
    11041 aatgcgcatt ctctctgctg agtcatctgg cgacagcagt tgagttcacc cgccaacaca
    11101 tgggcccagc tatgtagccg aaccctggct ctggaagtgc cagggacttt gtgcataagt
    11161 atgtaccatg cccttttttc acagtcctag ctctgcagaa gtgcagcctg aaggcctgtc
    11221 tgctgagagg acatgccctg gagccctgaa acaggcacag tgggaggagg aacggaggat
    11281 gacaggcatc aggccctcag tccaaaagca accacttgag aatgggctgg agtacgaaac
    11341 atggggtccc gtccctggat ccctcctcaa agagtaataa gtaaaatata aacaggtacc
    11401 ccaggccgtt ctgggtttgg gttgtaatgg gatccatttg cagagaacta ttgagacagc
    11461 ccagccgtac tgtgacaggc aatgtggggg aggaggttga atcacttggt atttagcatg
    11521 aatagaataa ttccctgaac atttttctta aacatccata tctaaattac caccactcgc
    11581 tcccagtctt cctgcctttg cgccagcctc ctgtctggcc atgcctgaag aaggctggag
    11641 aagccaccca cctcaggcca tgacactgcc agccacttgg caggtgcagc caaacctgag
    11701 ctgtcccaga aagggacatt ctcaagaccc aggcaccctg atcagcactg acttggagct
    11761 acaagtgtca tgccagaaaa gtctctaaga aaaccttttc agggaaaagg gggtgactca
    11821 acaccgggca agtttgggaa gccccaccct tcgagtgatg gaagagcaga taggaagcct
    11881 cagaagagag acaccggcac ccaggtaacg ttcctcatgt ggtctctgtc acacggtcga
    11941 ctctcttaag gtagctcgac cgtgctcttc cctggacatc tccgtgacca cactctcagt
    12001 tcttagggag atgcgggtgc tctctgaggc tatctcagag ttgcagattc tgaggcctag
    12061 agtgactaca gtcagcctag gaagccacag aggactgtgg accaggaggg cagaagagga
    12121 gaagggaaga aaaaccatca gataggactt gcaatgaaac taacccaaga caatcataat
    12181 gcagacagga atgttaaagg cgttcagcag ctggccatga cacccatctg tccctctggc
    12241 caagtcagca agcctggaag acctgggact cctgcccata tgtcctaagc tccccaccca
    12301 cccactcgtt cactgtcctt attctctctc taccttcagc cacttagttt cctaccttaa
    12361 gtcctagaat tgatcctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
    12421 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
    12481 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
    12541 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
    12601 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
    12661 accgtcatca ccgaaacgcg cga
//
