LOCUS       sequence-62866-e       13690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13690)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 13690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pThy1-Brainbow3.1
FEATURES             Location/Qualifiers
     source          1..13690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(6278..6311)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(6320..6353)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(6362..6395)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     regulatory      6404..6413
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6416..6436
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6437..7141
                     /codon_start=1
                     /product="Phialidium yellow fluorescent proteim"
                     /label=PhiYFP
                     /note="mammalian codon-optimized"
                     /translation="MSSGALLFHGKIPYVVEMEGNVDGHTFSIRGKGYGDASVGKVDAQ
                     FICTTGDVPVPWSTLVTTLTAGAQCFAKYGPELKDFYKSCMPDGYVQERTITFEGDGNF
                     KTRAEVTFENGSVYNRVKLNGQGFKKDGHVLGKNLEFNFTPHCLYIWGDQANHGLKSAF
                     KICHEITGSKGDFIVADHTQMNTPIGGGPVHVPEYHHMSYHVKLSKDVTDHRDNMSLKE
                     TVRAVDCRKTYL"
     polyA_signal    7324..7445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(7458..7491)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      7500..7509
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7506..8213
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    8413..8534
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(8551..8584)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      8593..8602
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8599..8601,8602..8604,8605..9315)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8599..8601,8602..8604,8605..9315)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8644..8665)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8905..8924)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9252..9273
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    9515..9636
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(9664..9697)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     regulatory      9706..9715
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9712..10407
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    10607..10728
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13521..13540)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13640..13662
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcaggatc gcggccgcga tccccgggcg agctcgaatt cagagaccgg
     2101 gaaccaaact agcctttaaa aaacataagt acaggagcca gcaagatggc tcagtgggta
     2161 aaggtgccta ccagcaagcc tgacagcctg agttcagtcc ccacgaacta cgtggtagga
     2221 gaggaccaac caactctgga aatctgttct gcaaacacat gctcacacac acacacacaa
     2281 atagtataaa caattttaaa tttcatttaa aaataatttg taaacaaaat cattagcaca
     2341 ggttttagaa agagcctctt ggtgacatca agttgatgct gtagatgggg tatcattcct
     2401 gaggacccaa aaccgggtct cagcctttcc ccattctgag agttctctct tttctcagcc
     2461 actagctgaa gagtagagtg gctcagcact gggctcttga gttcccaagt cctacaactg
     2521 gtcagcctga ctactaacca gccatgaaga aacaaggagt ggatgggctg agtctgctgg
     2581 gatgggagtg gagttagtaa gtggccatgg atgtaatgac cccagcaatg ctggctagaa
     2641 ggcatgcctc ctttccttgt ctggagacgg aacgggatca tcttgtactc acagaaggga
     2701 gaacattcta gctggttggg ccaaaatgtg caagttcacc tggaggtggt ggtgcatgct
     2761 tttaactcca gtactcagga ggcagggcca ggtggatctc tgtgagttca agaccagcct
     2821 gcactatgga gagagttttg ggacagccag agttacacag aaaaatcctg gtggaaaatc
     2881 tgaaagaaag agagaaagaa agaaagaaag aaagaaagaa agaaagaaag aaagaaagaa
     2941 agaaagaaag aaagaaagga agaaagaaag aaagagtggc aggcaggcag gcaggaggaa
     3001 ggaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa
     3061 aataggtgcg acttcaagat ccggagttac aagcagaatg cactgtttcc ctaacagggc
     3121 caagtgtttt gagtaactga aggtgggcat gatgcctggg aagcagaaac aagccaggca
     3181 gatgcacccc ttgccttgct tccgaagggc tgcagtagca tggaaaacat ggaaaacaac
     3241 caatccattc cctttgctga tataacaggc tccaaagcca aaacctgtca ctggaggctc
     3301 aagagcagat ctccagccaa gaggcaaagg aatgggggaa gctggagggc ctccctctgg
     3361 ttatccaggc ttctgaaggt tcaagcaaag aaagggttac aaccttaaaa ggagagcgtc
     3421 ccggggtatg ggtagaagac tgctccaccc cgacccccag ggtccctaac cgtcttttcc
     3481 ctgggcgagt cagcccaatc acaggactga gagtgcctct ttagtagcag caagccactt
     3541 cggacaccca aatggaacac ctccagtcag ccctcgccga ccaccccacc ccctccatcc
     3601 ttttccctca gcctccgatt ggctgaatct agagtccctc cctgctcccc cctctctccc
     3661 cacccctggt aaaactgcgg gcttcaggct gggtgcagca actggaggcg ttggcgacca
     3721 ggaggaggct gcagctaggg gagtccaggt gagagcaggc cgacgggagg gacccgcaca
     3781 tgcaaggacc gccgcagggc gaggatgcaa gccttcccca gctacagttt tgggaaagga
     3841 taccagggcg ctcctatatg ggggcgcggg aacctgggga aagaaggtgc tcccaggtcg
     3901 aggtgggaga ggaaggcagt gcggggtcac gggctttctc cctgctaacg gacgctttcg
     3961 aagagtgggt gccggagaga accatgagga aggacatcaa ggacagcctt tggtccccaa
     4021 gctcaaatcg ctttagtggt gcgaatagag ggaggaggtg ggtggcaaac tggagggagt
     4081 ccccagcggt gacctcgtgg ctggctggtg cggggcacca ggtaagaaac gcaatgttgc
     4141 gggaggggac tgggtggcag gcgcggggga ggggaaagct agaaaggatg cgagggagcg
     4201 gaggggggag ggagcggggg aatctcaact ggtagaggaa agttaaaatg aggaaatagc
     4261 atcagggtgg ggttagccaa gccgggcctc agggaaaggg cgcaaagttt gtctgggtgt
     4321 gggcttaggt gggctgggta tgagattcgg ggcgccgaaa acactgctgc gcctctgcca
     4381 aatcacgcta cccctgtatc tagttctgct aggcttctcc agccccagcc ccaattcttt
     4441 tctcagtgtc cccttccctc ccctgaatct caagcccaca ctccctcctc cataacccac
     4501 tgttatcaaa tctaagtcat ttgccaccca acaaccatca ggaggcggaa gcagacggga
     4561 ggagtttgag atcaacttgg gctacatcac gagttccaag ctcaccaagg cttcttaagg
     4621 agaccttgtc tctaaaatta attaattaat taattaatag tcccctttct ctgccacaga
     4681 accttgggat ctggctcctg gtcgcagctc cccccacccc aggctgacat tcactgccat
     4741 agcccatccg gaaatcctag tctattcccc atggatcttg aactgcagag agaatggcag
     4801 agtggcccgc cctgtgcaaa ggatgttcct agcctaggtg gagctcgcga actcggagac
     4861 tgtgcctctc ttgggcaagg acaggctaga cagcctgccg gtgttgagct agggcactgt
     4921 ggggaaggca gagaacctgt gcagggcacg caatgaacac aggaccagaa aactgcagcc
     4981 ctaggaacac tcaagagctg gccatttgca agcatctctg gcctccgtgc ttctcactca
     5041 tgtcccatgt cttatacagg cctctgtggc acctcgcttg cctgatctca tccctagccg
     5101 ttaagctttc tgcatgactt atcacttggg gcataatgct ggatacctac cattttctta
     5161 gaccccatca aaatcctatt tgagtgtacg gttcggagaa ctccttattt atccggtaaa
     5221 tgtcttttac tctgctctca gggagctgag gcaggacatt cctgagatac attgggagag
     5281 gaatacagtt tcaataaaat aataggttgg gtggaggtac atgcctataa tgccaccact
     5341 caggaaatgg tggcagcttc gtgagtttga ggccaaccca agaaacatag tgaaaccctg
     5401 tcagtaaata agtaagcaag tatttgagta tctactatat gctagggctg acctggacat
     5461 taggggtcat cttctgaaca aactagtgct tgagggaggt atttggggtt tttgtttgtt
     5521 taatggatct gaatgagttc cagagactgg ctacacagcg atatgactga gcttaacacc
     5581 cctaaagcat acagtcagac caattagaca ataaaaggta tgtatagctt accaaataaa
     5641 aaaattgtat tttcaagaga gtgtctgtct gtgtagccct ggctgttctt gaactcactc
     5701 tgtagaccag gctggcctgg aaatccatct gcctctgcct ctctgcctct ctgcctctct
     5761 gcctctctct ctgcctctct ctgcctctct ctgcccctct ctgcccctct ctgcccctct
     5821 ctgccgccct ctgccttttg ccctctgccc tctgttctct ggcctctgcc ctctccctgc
     5881 cttctccctc tgcctctgcc tcttgagtgc tggaatcaaa ggtgtgagct ctataggtct
     5941 taagttccag aagaaacgta atgaagtcac ccagcaggag gtgctcaggg acagcaagac
     6001 acacacaccc aggacactag gctcccactt ccttggcttt ctctgagtgg caaaggacct
     6061 taggcagtgt cactccctaa gagaagggga taaagagagg ggctgaggta ttcatcatgt
     6121 gctccgtgga tctcaagccc tcaaggtaaa tggggaccca cctgtcctac cagctggctg
     6181 acctgtagct ttccccacca cagaatccaa gtcggaactc ttggcaccta gaggatctcg
     6241 agcgtaacta taacggtcct aaggtagcga acctaggata acttcgtata gcatacatta
     6301 tacgaagtta tcggcgcgca taacttcgta taggatactt tatacgaagt tatcaagcgc
     6361 tataacttcg tatagtatac cttatacgaa gttatctgat atcgccacca tggctcctaa
     6421 gaagaagagg aaggtgatga gcagcggcgc cctgctgttc cacggcaaga tcccctacgt
     6481 ggtggagatg gagggcaatg tggatggcca caccttcagc atccgcggca agggctacgg
     6541 cgatgccagc gtgggcaagg tggatgccca gttcatctgc accaccggcg atgtgcccgt
     6601 gccctggagc accctggtga ccaccctgac cgcaggcgcc cagtgcttcg ccaagtacgg
     6661 ccccgagctg aaggatttct acaagagctg catgcccgat ggctacgtgc aggagcgcac
     6721 catcaccttc gagggtgatg gcaatttcaa gacccgcgcc gaggtgacct tcgagaatgg
     6781 cagcgtgtac aatcgcgtga agctgaatgg ccagggcttc aagaaggatg gccacgtgct
     6841 gggcaagaat ctggagttca atttcacccc ccactgcctg tacatctggg gcgatcaggc
     6901 caatcacggc ctgaagagcg ccttcaagat ctgccacgag atcaccggca gcaagggcga
     6961 tttcatcgtg gccgatcaca cccagatgaa tacccccatc ggcggcggcc ccgtgcacgt
     7021 gcccgagtac caccacatga gctaccacgt gaagctgagc aaggatgtga ccgatcaccg
     7081 cgataatatg agcctgaagg agaccgtgcg cgccgtggat tgccgcaaga cctacctgtg
     7141 aagcttaatt agctgagctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc
     7201 atctggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct
     7261 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     7321 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     7381 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7441 tcttaaggcg tgctagcata acttcgtata gcatacatta tacgaagtta tctaacgttg
     7501 ccaccatggt gagcaagggc gaggagaata acatggccat catcaaggag ttcatgcgct
     7561 tcaaggtgcg catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
     7621 agggccgccc ctacgagggc tttcagaccg ctaagctgaa ggtgaccaag ggtggccccc
     7681 tgcccttcgc ctgggacatc ctgtcccctc atttcaccta cggctccaag gcctacgtga
     7741 agcaccccgc cgacatcccc gactacttca agctgtcctt ccccgagggc ttcaagtggg
     7801 agcgcgtgat gaactacgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
     7861 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     7921 ccgtgatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
     7981 acggtgccct gaagggcaag atcaagatga ggctgaagct gaaggacggc ggccactaca
     8041 cctccgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     8101 tcgtcgacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     8161 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagagtgcac
     8221 gcgtgagtaa gctgaaccct cctgatgaga gtggccccgg ctgcatgagc tgcaagtgtg
     8281 tgctctcctg agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt
     8341 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     8401 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8461 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8521 atcaatgtat cttaaggcgt gtttaaacat ataacttcgt ataggatact ttatacgaag
     8581 ttatcgcgta cggccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     8641 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     8701 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     8761 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     8821 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     8881 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     8941 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     9001 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     9061 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     9121 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     9181 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     9241 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     9301 gacgagctgt acaagagtgc acgcgtgagt aagctgaacc ctcctgatga gagtggcccc
     9361 ggctgcatga gctgcaagtg tgtgctctcc tgagcggccg cgactctaga tcataatcag
     9421 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     9481 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     9541 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     9601 tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtgaagagct tcataatctt
     9661 catataactt cgtatagtat accttatacg aagttatcaa gtactgccac catggtgagc
     9721 gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt gaacaaccac
     9781 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga
     9841 atcaaggcgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc taccagcttc
     9901 atgtacggca gcaaaacctt catcaaccac acccagggca tccccgactt ctttaagcag
     9961 tccttccccg agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg
    10021 accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga
    10081 ggggtgaact tcccatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc
    10141 tccaccgaga ccctgtaccc cgctgacggc ggcctggaag gcagagccga catggccctg
    10201 aagctcgtgg gcgggggcca cctgatctgc aacttgaaga ccacatacag atccaagaaa
    10261 cccgctaaga acctcaagat gcccggcgtc tactatgtgg acagaagact ggaaagaatc
    10321 aaggaggccg acaaagagac ctacgtcgag cagcacgagg tggctgtggc cagatactgc
    10381 gacctcccta gcaaactggg gcacagaagt gcacgcgtga gtaagctgaa ccctcctgat
    10441 gagagtggcc ccggctgcat gagctgcaag tgtgtgctct cctgagcggc cgcgactcta
    10501 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
    10561 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
    10621 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    10681 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtactagc
    10741 tcgaggtcct tcctctgcag aggtcttgct tctcccggtc agctgactcc ctccccaagt
    10801 ccttcaaata tctcagaaca tggggagaaa cggggacctt gtccctccta aggaacccca
    10861 gtgctgcatg ccatcatccc ccccaccctc gcccccaccc ccgccacttc tccctccatg
    10921 cataccacta gctgtcattt tgtactctgt atttattcta gggctgcttc tgattattta
    10981 gtttgttctt tccctggaga cctgttagaa cataagggcg tatggtgggt aggggaggca
    11041 ggatatcagt cccctggggc gagttcctcc ctgccaacca agccagatgc ctgaaagaga
    11101 tatggatgag ggaagttgga ctgtgcctgt acctggtaca gtcatactct gttgaaagaa
    11161 tcatcgggga gggggggggg ctcaagaggg gagagctctc tgagcctttg tggaccatcc
    11221 aatgaggatg agggcttaga ttctaccagg tcattctcag ccaccacaca caagcgctct
    11281 gccatcactg aagaagcccc ctagggctct tgggccaggg cacactcagt aaagatgcag
    11341 gttcagtcag ggaatgatgg ggaaaggggt aggaggtggg ggagggatca ccccctcctc
    11401 taaaacacga gcctgctgtc tccaaaggcc tctgcctgta gtgagggtgg cagaagaaga
    11461 caaggagcca gaactctgac tccaggatct aagtccgtgc aggaagggga tcctagaacc
    11521 atctggttgg acccagctta ccaagggaga gcctttattc cttctttccc ttgcccctct
    11581 gtgccagccc ctcttgctgt ccctgatccc ccagacagcg agagtcttgc aacctgcctc
    11641 ttccaagacc tcctaatctc aggggcaggc ggtggagtga gatccggcgt gcacactttt
    11701 tggaagatag ctttcccaag gatcctctcc cccactggca gctctgcctg tcccatcacc
    11761 atgtataata ccaccactgc tacagcatct caccgaggaa agaaaactgc acaataaaac
    11821 caagcctctg gagtgtgtcc tggtgtctgt ctcttctgtg tcctggcgtc tgtctcttct
    11881 gtgttcttcc aaggtcagaa acaaaaacca cacacttcaa cctggatggc tcggctgagc
    11941 acttctgtgt gcagaaggtc caaccagact ctggggtacc ccggccctcc ctattccctt
    12001 gcctcctgtc tcccgctttt tatagctccc tatgctgggc ttctctggag agtgaaatct
    12061 ttgcccaaat caatgcgcat tctctctgct gagtcatctg gcgacagcag ttgagttcac
    12121 ccgccaacac atgggcccag ctatgtagcc gaaccctggc tctggaagtg ccagggactt
    12181 tgtgcataag tatgtaccat gccctttttt cacagtccta gctctgcaga agtgcagcct
    12241 gaaggcctgt ctgctgagag gacatgccct ggagccctga aacaggcaca gtgggaggag
    12301 gaacggagga tgacaggcat caggccctca gtccaaaagc aaccacttga gaatgggctg
    12361 gagtacgaaa catggggtcc cgtccctgga tccctcctca aagagtaata agtaaaatat
    12421 aaacaggtac cccaggccgt tctgggtttg ggttgtaatg ggatccattt gcagagaact
    12481 attgagacag cccagccgta ctgtgacagg caatgtgggg gaggaggttg aatcacttgg
    12541 tatttagcat gaatagaata attccctgaa catttttctt aaacatccat atctaaatta
    12601 ccaccactcg ctcccagtct tcctgccttt gcgccagcct cctgtctggc catgcctgaa
    12661 gaaggctgga gaagccaccc acctcaggcc atgacactgc cagccacttg gcaggtgcag
    12721 ccaaacctga gctgtcccag aaagggacat tctcaagacc caggcaccct gatcagcact
    12781 gacttggagc tacaagtgtc atgccagaaa agtctctaag aaaacctttt cagggaaaag
    12841 ggggtgactc aacaccgggc aagtttggga agccccaccc ttcgagtgat ggaagagcag
    12901 ataggaagcc tcagaagaga gacaccggca cccaggtaac gttcctcatg tggtctctgt
    12961 cacactaggt gctcttccct ggacatctcc gtgaccacac tctcagttct tagggagatg
    13021 cgggtgctct ctgaggctat ctcagagttg cagattctga ggcctagagt gactacagtc
    13081 agcctaggaa gccacagagg actgtggacc aggagggcag aagaggagaa gggaagaaaa
    13141 accatcagat aggacttgca atgaaactaa cccaagacaa tcataatgca gacaggaatg
    13201 ttaaaggcgt tcagcagctg gccatgacac ccatctgtcc ctctggccaa gtcagcaagc
    13261 ctggaagacc tgggactcct gcccatatgt cctaagctcc ccacccaccc actcgttcac
    13321 tgtccttatt ctctctctac cttcagccac ttagtttcct accttaagtc ctagaattga
    13381 tcctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
    13441 aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
    13501 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
    13561 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
    13621 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
    13681 aaacgcgcga
//
