LOCUS       sequence-62883-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Scholz J, Besir H, Strasser C, Suppmann S
  TITLE     A new method to customize protein expression vectors for fast, 
            efficient and background free parallel cloning.
  JOURNAL   BMC Biotechnol. 2013 Feb 14;13(1):12.
  PUBMED    23410102
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-02-14"; volume: "13(1)"; pages: "12"
COMMENT     Sequence Label: pCoofy40
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     458..477
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             521..544
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     RBS             581..589
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             591..899
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MIQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     778..797
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ctgcgagggc cagctgttgg gctcgcggtt gaggacaaac tcttcgcggt ctttccagta
       61 ctcttggatc ggaaacccgt cggcctccga acggtactcc gccaccgagg gacctgagcg
      121 agtccgcatc gaccggatcg gaaaacctct cgagaaaggc gtctaaccag tcacagtcgc
      181 aaggtaggct gagcaccgtg gcgggcggca gcgggtggcg gtcggggttg tttctggcgg
      241 aggtgctgct gatgatgtaa ttaaagtagg cggtcttgag acggcggatg gtcgaggtga
      301 ggtgtggcag gcttgagatc cagctgttgg ggtgagtact ccctctcaaa agcgggcatt
      361 acttctgcgc taagattgtc agtttccaaa aacgaggagg atttgatatt cacctggccc
      421 gatctggcca tacacttgag tgacaatgac atccactttg cctttctctc cacaggtgtc
      481 cactcccagg tccaagttta aacggatctc tagcgaattc ctggaagttc tgttccaggg
      541 gcccttgaca acataaaaac tttgtgttat acttgtaacg taaggaggta atgattcagt
      601 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
      661 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
      721 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
      781 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
      841 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataag
      901 a
//
